依达拉奉对断肢再植后缺血再灌注损伤大鼠骨骼肌线粒体的保护作用及机制.pdfVIP

山东医药2007 年第47 卷第9 期 　 依达拉奉对断肢再植后缺血再灌注损 大鼠骨骼肌线粒体的保护作用及机制 1* 2 1 1 3 2 2 2 , , , , , , , ( 1 ,510515;2 ;3 ) [ ]　探讨依达拉奉对断肢再植后缺血再灌注损 大鼠骨骼肌线粒体的保护作用及其机制。 36 只成年Wistar 大鼠, 随机分为对照组、冷冻组和药物组。对照组只暴露股动、静脉, 不离断肢体; 余两组制作 深低温保存断肢再植缺血再灌注损 动物模型, 药物组洗脱液中含依达拉奉0.5 mg/ kg。选取相同时点骨骼肌标 本, 提取骨骼肌线粒体, 测定线粒体丙二醛(MDA) 、超氧化物歧化酶(SOD) 、线粒体呼吸控制率及抗氰呼吸, 并对 骨骼肌线粒体超微结构行透射电镜观察。与对照组相比, 冷冻组与药物组线粒体中的MDA 显著增高, 抗氰 呼吸显著增加, SOD 显著降低, 呼吸控制率显著下降。与冷冻组比较, 药物组的MDA 水平及抗氰呼吸显著降低, SOD 显著增高, 呼吸控制率显著升高( P 均 0.05);病理改变在冷冻组最为严重, 药物组再灌注骨骼肌大部分线粒体完 整。依达拉奉能减轻深低温保存大鼠断肢再植骨骼肌线粒体的缺血再灌注损 。其可能与依达拉奉直接抑制羟 自由基, 提高骨骼肌线粒体内SOD 活性, 减少MDA 的产生, 使细胞进行正常的氧化磷酸化有关。 [ ]依达拉奉; 缺血再灌注损 ;线粒体;低温保存; 断肢再植术 [ ] 318.52; 658. 1[] [ ]1002-266 ( 2007)09-0015-03 R R A X Protec tive effect of e da rav one on the ischemia rep erfusion inj ury of mitochondrium in rep la nt ed rat e xt re mit ie s 1 DUAN Yong-zhuang , WANG Zeng -tao,ZH ONG Shi-zhen,DIN G Zi -hai, - , - , , FU Qing lin H ao Li wen H e Bo Miao Bo ( 1Southern Me ical University , Guang zhou 510515 P .R. China ) Ab str act :[Objective] T o explore the protective effect and mechanism of edaravone on the mitochondrium of replanted rat limb. [Methods] 36 adult Wistar rats were divided into 3 groups randomly control group cold: , storage group and study group, In the control group, the femoral artery and vein was exposed only without ligation.The model of rats hind limb skelet al muscle with ischemia reperfusion injury under profound hypothermia was established in the cold st orage group and experimental group In the study group the perfusate. , contains edaravone 0. 5mg/ kg. The level of MDA, SOD、RCR and cyanide-resistant of mitochondria, cyanid