细胞表面唾液及其连接方式对乳腺癌细胞黏附的影响.docVIP

细胞表面唾液酸及其连接方式对乳腺癌细胞黏附的影响 作者：王晓玉1；林绍强2；李君武2；Wolfgang Kemmner3；丁彦青4　　(暨南大学1第一医院妇产科，2医学院微生物与免疫学教研室，广东??广州??510630；3Max-Delbrueck Center for Molecular Medicine，Berlin 13125, Germany；4南方医科大学肿瘤学研究所，广东??广州??510515) 摘要：目的??研究肿瘤细胞总唾液酸及α2，6-连接唾液酸对乳腺癌细胞MDA-MB435黏附力的影响。方法??乳腺癌细胞MDA-MB-435转染顺义或反义α2，6唾液酸转移酶(ST6Gal-I) cDNA，以黑接骨木凝集素SNA筛选高、低表达α2，6唾液酸细胞克隆，检测各转染细胞的同源凝集作用，并比较唾液酸酶处理前后肿瘤细胞对胶原IV黏附力的变化。结果 顺义转染克隆细胞-细胞之间的同源黏附能力下降，与胶原IV的黏附增强；反义转染克隆细胞-细胞之间同源黏附能力增强，而与胶原IV的黏附降低。唾液酸酶处理后肿瘤细胞与胶原IV的黏附力下降至未处理前的31%～57%。 结论??乳腺癌MDA-MB435细胞表面唾液酸及α-2，6唾液酸化对肿瘤细胞的黏附有重要影响。 关键词：乳腺肿瘤；唾液酸转移酶；细胞外基质；细胞黏附；肿瘤转移 中图分类号：R737??文献标识码：A??文章编号：1673-4254(2006)06-0742-05 Effect of cell surface sialic acid and their linkages on adhesion of mammary carcinoma cellsWANG Xiao-yu1； LIN Shao-qiang2； LI Jun-wu2； Kemmner Wolfgang3； DING Yan-qing4Department of Obstetrics and Gynecology, First Affiliated Hospital of Ji’nan University1, Department of Microbiology and Immunology of Medical College2, Jinan University, Guangzhou 510630, China; 3Max-Delbrueck Center for Molecular Medicine，Berlin 13125, Germany; 4Institute of Oncology, Southern Medical University, Guangzhou 510515, ChinaAbstract: Objective??To investigate the effect of cell surface sialic acid and its linkage on the cell-cell and cell-matrix adhesion of mammary carcinoma cells MD-MB-435. Methods MD-MB-435 cells were sense-transfected with ST6Gal I cDNA or antisense-transfected with part of the ST6Gal I sequence inserted in pcDNA 3.1 vector, with mock transfection with pcDNA3.1 vector as the control. The cell surface α2, 6-linked sialylation was determined by fluorescence-activated cell sorting (FACS) using lectin SNA (Sambucus nigra agglutinin specific to α2, 6-linked sialic acid on N-linked glycoprotein). A significantly increased α2, 6-sialylation subclone in sense-tranfectants and a decreased α2, 6-sialylation subclone in antisense-transfectants were selected for further examination of cell-cell and cell-matrix (collagen IV) adhesion. The transfectants were also