水稻内生细菌群落的生物多样性的研究 - yzxzcom.doc

问题： 根系改为根系 将根系细菌鉴定相似指数0.5的排出在分析之外。 最重要的问题是如何保证细菌鉴定的准确性。 水稻根系细菌和内生细菌与水稻生物学特性间的相关系数原始数据要列出，表1放到后面。 根系细菌和内生细菌在不同品种水稻的分布特性要分别分析 作根系和内生的关系。 内生菌和根系细菌与生物学特性的关系， 过来讨论， 水稻根系及内生细菌群落结构多样性研究 胡桂萍1,2 刘波* 郑雪芳 林营志史怀尤民生* (1福建省农林大学应用生态研究所，福建省福州市，350002；福建省农业科学院农业生物资源研究所，福建省福州市，350003) 为探讨水稻根系细菌和内生细菌群落结构多样性及与水稻的关系。2008年7月从5个品种水稻根系土和茎部分离根系细菌和内生细菌并脂肪酸鉴定。结果表明，分离得到的根系细菌有13个种，于10属，内生细菌有13个种，属于9属，两的优势菌为蜡状芽孢杆菌（Bacillus cereus）。以各菌株在根系土和茎部出现与否为指标，用类平均法对数据进行聚类分析，，。对根系细菌、内生细菌和水稻生物学特征参数进行相关分析得到，根系细菌和内生细菌与水稻千粒重，穗粒数，亩产存在正相关有效穗。 Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China ;2 Agricultural Biological Resource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China) Abstract：The research aimed to study the relationship between the diversity of rhizosphere bacteria and endophytic bacteria and rice. We isolated and cultured rhizosphere bacteria and rhizosphere bacteria from the rhizosphere and stem of rice. fatty-acid identification showed that rhizosphere bacteria were clustered into 10 genera which obtained 13 species and rhizosphere bacteria were clustered into 9 genera which obtained 13 species, The dominant bacteria is Bacillus cereus. The cluster analysis results showed that the rhizosphere bacteria could be separated into 4 groups, whereas the endophytic bacteria can be separated into 3 groups. The correlation analysis indicated the relationship between bacteria and Seed setting percentage、1000-grain weight and plant production were positively correlated Wand effective spikes was negatively correlated. Key words：rice, Rhizosphere bacteria, Endophytic bacteria, Diversity of community 植物内生细菌（Endophytic bacteria）是定殖在植物。掘出掘出的植株土壤部分严密的用塑料袋包，带回实验室表1 水稻品种生物学特性 Table 1 biological charateristics for rice varieties品种全生育期穗瘟(﹪) 最高分蘖数有效穗成穗率穗粒数结实率千粒重亩产(kg) 株高(cm) 航1号 133.67 31.16 12.13 8.91 73.44 122.29 78.52 28.11 485.44 117.67 航2号 133.67 7.66 14.49.30 64.49 126.63 76.81 28.23 519.93 116.67 Ⅱ优航1号 130.67 84.26 14.03 8.359.51 127.5