Zebrafish twist1 is expressed in craniofacial, vertebral, and renal precursors.教程.pdfVIP

Dev Genes Evol (2007) 217:783–789 DOI 10.1007/s00427-007-0187-7 SHORT COMMUNICATION Zebrafish twist1 is expressed in craniofacial, vertebral, and renal precursors Gare-Hoon Yeo Felicia S. H. Cheah Ethylin Wang Jabs Samuel S. Chong Received: 3 May 2007 /Accepted: 18 September 2007 / Published online: 11 October 2007 # Springer-Verlag 2007 Abstract TWIST1 encodes a transcription factor that vation at the gene structure, nucleotide, and predicted contains a highly conserved basic helix–loop–helix DNA- peptide levels across large evolutionary distances. Using binding domain and a WR motif. We have isolated a full- reverse-transcription polymerase chain reaction analysis length complementary DNA of the zebrafish ortholog of and in situ hybridization analyses of whole mount and TWIST1 and determined its genomic organization. Inter- cryosectioned zebrafish embryos, we detected maternal species comparisons reveal a remarkable degree of conser- twist1 transcript in the zygote. During somitogenesis, twist1 transcripts were detected in the intermediate mesoderm At the time of acceptance of this manuscript, a publication entitled from the 2-somite to 18-somite stages, followed by “Four twist genes in zebrafish, four expression patterns” by expression in the somites from the 5-somite stage to the Germanguz et al. appeared in the August 2007 issue of Developmental 24-somite stage. Also, beginning at the two-somite stage, Dynamics. twist1 expression was observed in head mesenchyme and, Communicated by M. Hammerschmidt