EGF对不同直径卵泡绵羊卵母细胞体外成熟、受精及胚胎发育影响.pdfVIP

摘 要 为了研究EGF对绵羊不同直径卵泡卵母细胞体外成熟和早期胚胎发育的影响，通过9个系列试 验，比较不同直径卵泡特征，卵母细胞的质量、核型、成熟率和早期胚胎发育能力，并在成熟液和 发育液中添加100ng/mlEGF，通过成熟率、胚胎发育能力及囊胚细胞总数，研究EGF对不同直径卵泡 卵母细胞体外成熟和胚胎发育的影响。 1. 新采集方法-刺剖法能明显提高绵羊采卵数量，且卵泡直径越大，卵母细胞的A 、B 级比例也越 高。 2. LH 、E2 的同一种工作浓度在不同季节对绵羊卵母细胞的成熟率是不同的。 3. 卵泡直径越大，卵母细胞的成熟率也越高，且成熟受精后，胚胎发育能力也正常。 4. EGF 对生发泡破裂（GVBD ）具有积极的影响，通过卵母细胞的促成熟作用明显改善胚胎发育 率，尤其是偏势卵泡卵母细胞形成的胚胎，胚胎培养液中添加EGF对囊胚细胞总数有积极的影 响。 关键词：表皮生长因子，绵羊卵母细胞，体外成熟，胞质成熟，核型，囊胚率 I ABSTRACT To investigate the effect of epithelium growth factor on the different size follicle of ovine oocytes maturation and the early development of embryo in vitro ,a series of nine experiments were conduted to compare characteristic of different size follicle 、the grade of different size follicle of oocyte 、its chromatin configuration 、maturation and the early development of embryo in vitro, 100ng/mlEGF was supplemented to the maturation medium 、the culture medium respectively. Finally to reveal the effect of EGF on the ovine oocytes maturation and the early development of embryo in vitro through maturation rate、embryo development competence and the cell numbers of blastocsts. The conclusion were following: 1.the new collection method of cuting and scraping can significant increase the total of cumulus-oocyte complexes of ovine and the follicle diameter was bigger The percentage of grade A and B was higher. 2.The maturation rate of ovine oocyte was different with the same woking concentration of LH、E2 on breeding season and nonbreeding season.. 3. the follicle diameter was bigger, the maturation rate was higher, and the trend of early development competence of embryo was normal. 4.EGF effect the Germinal Vesicle Breakdown positively,the development competence of embryo was improved by the supplement epithelium g