血清生物标记者发现途径.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2010, Article ID 927917, 8 pages doi:10.1155/2010/927917 Methodology Report A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation Emilie Ernoult, Anthony Bourreau, Erick Gamelin, and Catherine Guette Centre INSERM Regional de Recherche sur le Cancer U892, Centre Regional de Lutte Contre le Cancer Paul Papin, ´ ´ 2 rue Moll, 49933 Angers Cedex 9, France Correspondence should be addressed to Catherine Guette, c.guette@unimedia.fr Received 4 June 2009; Revised 17 July 2009; Accepted 3 August 2009 Academic Editor: Pieter C. Dorrestein Copyright © 2010 Emilie Ernoult et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Human blood plasma contains a plethora of proteins, encompassing not only proteins that have plasma-based functionalities, but also possibly every other form of low concentrated human proteins. As it circulates through the tissues, the plasma picks up proteins that are released from their origin due to physiological events such as tissue remodeling and cell death. Specific disease processes or tumors are often characterized by plasma “signatures,” which may become obvious via changes in the plasma proteome profile, for example, through over expression of proteins. However, the wide dynamic range of proteins present in plasma makes their analysis very challenging, because high-abundance proteins tend to mask those of lower abundance. In the present study, we used a strategy combining iTRAQ as a reagent which