文献_BGI_Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm.pdfVIP

Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm Yong Hou,1,2,3,11 Luting Song,1,4,5,6,11 Ping Zhu,7,11 Bo Zhang,1,11 Ye Tao,1,11 Xun Xu,1 Fuqiang Li,1 Kui Wu,1 Jie Liang,1 Di Shao,1 Hanjie Wu,1 Xiaofei Ye,1 Chen Ye,1 Renhua Wu,1 Min Jian,1 Yan Chen,7 Wei Xie,1,3 Ruren Zhang,1,3 Lei Chen,1,4,5,6 Xin Liu,1 Xiaotian Yao,1 Hancheng Zheng,1 Chang Yu,1 Qibin Li,1 Zhuolin Gong,1 Mao Mao,8 Xu Yang,1 Lin Yang,1 Jingxiang Li,1 Wen Wang,5 Zuhong Lu,2,3 Ning Gu,2,3 Goodman Laurie,1 Lars Bolund,1 Karsten Kristiansen,1,9 Jian Wang,1 Huanming Yang,1 Yingrui Li,1,* Xiuqing Zhang,1,* and Jun Wang1,9,10,* 1BGI-Shenzhen, Shenzhen, 518083, China 2State Key Laboratory of Bioelectronics 3School of Biological Science and Medical Engineering Southeast University, Nanjing 210096, China 4College of Life Sciences, Wuhan University, Wuhan 430072, China 5CAS-Max Planck Junior Research Group, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences (CAS), Kunming, Yunnan 650223, China 6Graduate School of Chinese Academy of Sciences, Beijing 100049, China 7Department of Hematology, Peking University First Hospital, Beijing 100034, China 8Pfizer Inc., San Diego, CA 92121, USA 9Department of Biology 10The Novo Nordisk Foundation Center for Basic Metabolic Research University of Copenhagen, DK-1165 Copenhagen, Denmark 11These authors contributed equally to this work *Correspondence: wangj@ (J.W.), zhangxq@ (X.Z.), liyr@ (Y.L.) DOI 10.1016/j.cell.2012.02.028 SUMMARY INTRODUCTION Tumor heterogeneity presents a challenge for infer- Tumor evolution is an important area in cancer research because ring clonal evolution and driver gene identification. information on fundamental genetic changes that occur as Here, we describe a method