IL-17+ T 细胞及其相关细胞因子在香烟烟熏致小鼠呼吸道炎症中的变化和意义.docVIP

IL-17+T细胞及其相关细胞因子在烟熏致小鼠呼吸道炎症中的变化和意义 目的 观察IL-17+T淋巴细胞及相关细胞因子在烟熏导致的呼吸道炎症中的变化Th17、γδT细胞等方法 采用随机数字表法将24只C57/BL6小鼠分为4组对照组（C）烟熏10（CS10）、20（CS20）30 d（CS30）；苏木精-伊红染色（HE）观察肺组织病理变化； ELISA法检测血清和支气管肺泡灌洗液（bronchoalveolar lavage fluid，BLAF）中相应细胞因子IL-17、IL-22和IL-23的表达水平。流式细胞术检测分泌IL-17的Th17细胞和γδT细胞比例。结果 随着烟熏时间的延长，小鼠肺组织中淋巴细胞浸润增多、上皮细胞倒伏及支气管壁变厚增生；IL-17、IL-22和IL-23表达量在各烟熏组之间差异显著（P0.05）；Th17、IL-17+ γδT细胞比例较对照组都有不同程度的增高（P0.05）。结论 IL-17+T细胞及其相关细胞因子可能是烟熏导致的支气管炎症的始发因素之一，其含量的变化对支气管炎的发展和预后都具有重要提示作用。 [关键词] Th17细胞；γδT细胞；IL-17+γδT细胞；烟熏刺激；支气管炎[中图法分类号] [文献标志码] A Change and significance of IL-17+T cells and related cytokine in mouse airway inflammation which induced by cigarette smoking Zhang Peng, Liu Tao, Liu Gang()？ [Abstract] Objective To observe the change of IL-17+T cells and related cytokines in airway inflammation which induced by cigarette smoking, to explain the relationship between them. Methods A total of 24 healthy C57/BL6 mouse were randomly and equally divided into control group, CS(cigarette smoking)10 days group,CS20 days group and CS30days group; Pathological change of lung was observed by hematoxylin–eosin stain; The concentration of IL-17、IL-23 and IL-22 which collected from serum and bronchoalveolar lavage fluid (BLAF) was detected by enzyme-linked immunosorbent assay ( ELISA); The frequencies of subtype of IL-17+T cell was detected by Flow Cytometry(FCM). Results Compare with control groups the lung have different pathological change included lymphocytes infiltration increase, epithelium lodging, bronchus thicken ; The concentration of IL-17, IL-22 and IL-23 in the cigarette smoking groups was higher than that of the control groups( P 0.05) . The frequencies of subtype of IL-17+T cell IL-17+γδT also was also higher than that of the control groups (p0.05).Conclusion IL-17+T and related cytokine is the beginning factors of bronchitis in possible, the quantitative change of them have a important associated with bronchitis develop