茉莉花植株愈伤组织的诱导及其继代培养保持（.docVIP

茉莉花愈伤组织的诱导及其继代( 甘煌灿，赖呈纯，朱菁李延晖，（ 350108；2. 福建省农业科学院农业所福建 福州 350003福建省农业科学院农业工程技术研究所，福建 福州 350003，福建 福州 350） 摘要：以福州茉莉[Jasminum sambac (Linn.) Aiton] 茎段或花为外植体，进行其愈伤组织诱导、继代增殖和长期保持等方面的研究。结果表明，在光照条件为1500-2000 lx下，能够获得6种类型的愈伤组织，愈伤组织诱导适宜培养基为MS+BA 2.0 mg·L-1+NAA 0.2 mg·L-1+蔗糖30 g·L-1，诱导率可达100%；MS+BA 2.0 mg·L-1+NAA 0.2 mg·L-1+蔗糖30 g·L-1，。 关键词：茉莉；外植体；愈伤组织；诱导；继代培养 中图分类号： 文献标识码： 文章编号： Induction of Fu jasmine (Jasminum sambac (Linn.) Aiton) and Its Subculture and Maintenance Gan Huang-can1, Lai Cheng-chun3, Zhu Yu-jing2, Chen Wen-hui4, Li Yan-Hui3, Liu Bo2,* （1. College of Biological Sciences and Technology, Fuzhou University, Fuzhou，Fujian 350108China; 2. Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 3. Institute of Agricultural Engineering and Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 4. Institute of Vegetable Crops, Fuzhou 350111, China) Abstract：The plantlets of Fu jasmine [Jasminum sambac (Linn.) Aiton] were established by using its stems or floral organ as explants. In this paper, its callus induction, subculture and long-term maintenance were vaccinated on media with different concentrations of phytohormone. The results indicated that the suitable conditions for callus growth were light densities of 1500-2000 lx and different phytohormones could form six types of callus. The medium of MS+BA 2.0 mg·L-1+NAA 0.2 mg·L-1+ sucrose 30 g·L-1 was the best for callus inducing, with an average induction rate of 100%. The medium of MS+BA 2.0 mg·L-1+NAA 0.2 mg·L-1+ sucrose 30 g·L-1 was the best for the callus subculture multiplication, and the callus with good growth state could be obtained. Key words：jasmine (Jasminum sambac (Linn.) Aiton); explant; callus; induction; subculture and maintenance 茉莉花 [Jasminum?sambac (Linn.) Aiton] 为木犀科素馨属（或茉莉属）[1]亚热带常绿灌木。茉莉花树冠直立或半开展状，花冠白色，极芳香，花期较长[2,3]；《本草纲目》中记载其花、叶、根均可入药，根能理气、活血、安神镇痛，花能开郁、辟秽，叶能消肿解毒；近代研究也表明，茉莉花根提取物具有抗心律失常、镇静催眠、抑制中