支气管哮喘患者诱导痰中白细胞介素-9水平及其与气道炎症的关系-论文.docVIP

支气管哮喘患者诱导痰中白细胞介素-9水平及其与气道炎症的关系 　【摘要】 目的 探讨支气管哮喘患者诱导痰中白细胞介素9(IL9)的水平及其与炎症细胞和肺功能的关系。方法 分别选择28例支气管哮喘急性发作患者(哮喘急性发作组)和28例健康对照(健康对照组),分别检测哮喘急性发作期和治疗后缓解期及健康对照组的肺功能;并用酶联免疫吸附实验(ELISA)测定诱导痰上清液中IL9的水平。 结果 哮喘发作组患者诱导痰中IL9水平明显高于哮喘缓解期,比较差异有统计学意义(P005),哮喘发作组和缓解期组痰中IL9水平高于健康对照组,比较差异有统计学意义(P005)。哮喘发作组患者诱导痰中IL9水平与中性粒细胞数及嗜酸性粒细胞数均 　　呈正相关(r值分别为068、081, P005),与第一秒用力呼气容积占预计值的百分比(FEV%)呈负相关(r=065,P005)。结论 支气管哮喘患者诱导痰中增高的IL9水平与其气道炎症和气流受限有关。 　　【关键词】 支气管哮喘;白细胞介素9;气道炎症 　　The levels of interleukin 9 in induced sputum in patients with asthma and its relationship to airway inflammation 　　SUN Wangyuan.Department of Respiratory,Chinese Medicine of Suqian,Jiangsu 223800,China 　　【Abstract】 Objective To investigate the levels of interleukin9(IL9)in induced sputum in patients with asthmaand its relationship to the number of inflammation cells and lung function Methods 28 patients with asthma and 28 normal control subjects were included in this studyLung function was measuredInduced sputum was obtained and processed for cell differential and the supernatant was assayed for the concentrations of IL9 by ELISA Results The levels of IL9 in induced sputum of the asthmatics were significantly higher than those in the controls and in normal control subjects(P005) In the acute exacerbated asthmatics, a positive correlation was found between the level of IL9 and the percentage of neutrophile(r=068,P005), but the level of IL9 correlated inversely with FEV1%(r=065,P005) Conclusion Increased levels of IL9 in induced sputum in patients with asthma(especially in exacerbation) are associated with airway inflammation and airflow limitation 　　【Key words】 　　Asthma; Airway inflammation; Interleukin9 　　 支气管哮喘(哮喘)已被公认是多种炎症细胞及结构细胞及其组分参与并伴有气道高反应性的一种慢性气道特应性炎症性疾病。白细胞介素9(IL9)是一种由激活的辅助性T细胞分泌的细胞因子[1],研究发现IL9可广泛作用于包括支气管哮喘在内的I型变态反应中,其对T细胞、肥大细胞、嗜酸粒细胞、气道上皮细胞等的生长增殖分化、细胞的功能、细胞间信息传递等都具有重要的调节作用。本研究测定IL9在哮喘患者气道中的表达,以探讨IL9在哮喘发病机制中的作用。 　　1 资料和方法 　　11 一般资料 　　选择本院2008年7月至2009年9月门诊就诊的2