MicroRNA及其在人和动物上研究进展.pdfVIP

HEREDITAS (Beijing) 2007 6 , 29(6): 651―658 ISSN 0253-9772 综述 DOI: 10.1360/yc-007-0651 MicroRNA 盛熙晖, 杜立新 , 100094 : MicroRNA 是一种长约 22nt 的非编码 RNA, 通过与靶基因的 3′UTR 区结合来调控靶基因的表达。目前 已证实 miRNA 在生物体生长、发育和疾病发生等过程中发挥着重要的作用。文章介绍了 miRNA 的特征、作 用机制, 综述了关于 miRNA 的功能、miRNA 基因的鉴定与靶基因预测的最新研究进展。 : microRNA; siRNA; 基因鉴定; 发育; 疾病 Progress on the research of microRNAs and its function in humans and animals SHENG Xi-Hui , DU Li-Xin Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China Abstract: MicroRNAs, a class of noncoding RNA of approximately 22 nucleotides, can regulate gene expression by bind- ing to the regin of 3′ UTR of the target mRNAs. To date,it has been demonstrated for organism that miRNAs play an im- portant role in growth,development,and occurrence of disease. This paper introduces the character and the active mecha- nism of miRNAs,and the newest progress in the research on the function of microRNA,the identification of microRNA gene and the prediction of target gene also are summarized here. Keywords: microRNA; siRNA; identification of genes; development; disease MicroRNAs (miRNAs) 22nt miRBase Release 9.1: 2007.12.at http://microRNA.san RNA, ger.ac.uk)miRNA [1], mRNA , 3′ (3′UTR), , miRNA miRNA1993 , Lee[2] miRNA , (C.elegans) , lin-42000 , Reinhart [3] RNA: let-7 , RNA, 1 microRNA RNA 1.1 microRNA microRNA(miRNA)[4] , 4,449 miRNA, MicroRNARNA, 474 ( [5]: , Northern RNA 收稿日期: 2006− 10−30; 修回日期: 2006− 12−06 作者简介: (1983−), , , , : Tel: 010 E-mail: sxh03@ 通讯作