量子点增强CE-LIF测定BPDE-DNA加合物及其应用刘胜权王智鑫.docVIP

量子点增强CE-LIF测定BPDE-DNA加合物及其应用 刘胜权 王智鑫 汪海林* 中国科学院生态环境研究中心，环境化学与生态毒理学国家重点实验室，100085，北京 E-mail：hlwang@rcees.ac.cn 苯并芘（B[a]P）在生物体内代谢活化形成7，8-二-9，10-环氧化苯并芘Quantum Dot enhanced CE-LIF for the determination of BPDE-DNA adduct and its applications Sheng-Quan Liu，Zhi-Xin Wang，Hai-Lin Wang* State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, 100085, Beijing Benzopyrene (B[a]P) can be metabolized and activated to 7,8-diol-9,10-epoxy-benzopyrene in vivo，which can react with DNA bases and form BPDE-DNA adduct，which is an important biomarker. Here, we used the QD enhanced CE-LIF (Capillary electrophoresis-Laser induced fluorescence) developed by our laboratory and studied DNA damage resulted from exposure of B[a]P and BPDE and cell’s repair capacity. That is：Dose-effect relationship between exposure concentration of B[a] P and BPDE-DNA adduct and the change of repair capacity of mice’s ES cell when a significant repair gene is knocked out. Fig.2 Different repair capacity between three styles of ES cells through the determination of BPDE-DNA adduct using QD enhanced ultrasensitive CE-LIF immunoassay Fig.1 dose-effect relationship between exposure concentration of BaP and BPDE-DNA adduct using QD enhanced ultrasensitive CE-LIF immunoassay