气相色谱前,脂肪酸甲酯.pptVIP

Journal of Lipid Research Volume 51, 2010 635-40;Preparation of fatty acid methyl esters for gas-liquid chromatography; 1 Introduction;1.1 Base-catalyzed Base-catalyzed methanolysis proceeds much more rapidly than acid catalyzed reactions but cannot catalyze the esterification of FFAs.; In this study, we have developed a convenient reagent for preparation of FAMEs from acyl lipids, including SEs, triacylglycerols (TGs), phospholipids (PhLs), and FFAs. The reagent is composed of commercial concentrated HCl (conc. HCl), methanol, and toluene and is superior to ot