Citricacidcycle：柠檬酸循环.docVIP

University of Cape Town Department of Molecular and Cell Biology Course Code: MCB2015S Dr J Rodrigues Course Name: Introduction to Metabolism Test 1 August 2008 (Total = 60 marks) (note that multiple choice questions will be subjected to negative marking) Overview of Metabolism (15 marks) 1. Calculate the overall (G°’ value for the ATP-coupled reactions below (see data sheet for (G°’ values): phosphocreatine + ADP ( creatine + ATP (2) ATP + glucose ( ADP + glucose 6-phosphate (2) 2. Calculate the free energy change for acetyl phosphate hydrolysis in a solution of 2 mM acetate, 2 mM phosphate, and 3 nM acetyl phosphate. Acetyl phosphate undergoes the following reaction: Acetyl-P + H2O ( acetate + Pi (6) MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question (questions 3 - 7 ). 3. If a chemical reaction starts with 1 M concentrations each of reactants A and B and products C and D, under what conditions of Keq and ΔGo will the reaction proceed in the forward direction (ΔGo = -RT ln Keq)? (A) If Keq is greater than 1 and ΔGo is positive. (B) If Keq is less than 1 and ΔGo is positive. (C) If Keq is greater than 1 and ΔGo is negative. (D) If Keq is negative and ΔGo is negative. (E) If Keq is 0 and ΔGo is positive. (1) 4. When a nucleophile attacks the α-phosphorus atom of ATP, what kind of transfer occurs? (A) pyrophosphoryl transfer (B) phosphoryl transfer (C) adenine transfer (D) adenosine transfer (E) cyclic-AMP transfer (1) 5. High-energy compounds exhibit large negative free energy of hydrolysis and include all EXCEPT: (A) phosphate esters. (B) enol phosphates and phosphate anhydrides. (C) acyl phosphates. (D) guanidino phosphates. (E) thioesters. (1) 6. ATP hydrolysis coupled reactions have equilibrium constants that are changed by a factor of: (A) 10 (B) 100 (C) 104 (D) 106 (E) 108 (1) 7. The hydrolysis of a phosphate from which of the following m