GelElectrophoresisProblems-Wikispaces：凝胶电泳-wiki空间问题.docVIP

Gel Electrophoresis Problems Problem 1 Rape Investigation Blood samples are drawn from a rape victim and a suspect. A few hours after the crime the, victim went to a hospital where she was examined. A vaginal swab collected evidence of the crime. You are on the jury. Is the defendant guilty or not guilty? Why? Problem 2 Who is the father? DNA electrophoresis of a man and his four children is shown at the left. Which lane is the father? Why? Problem 3 Crime Scene The gel on the left shows comparisons of DNA fingerprints from a bloodstain discovered at a crime scene and from blood samples of seven suspects. Which suspect matches the DNA at the crime scene? Why? Problem 4 #1 #2 #3 #4 In this imaginary paternity test, four autoradiographs (similar test to gel electrophoresis test) show DNA “fingerprints” taken from each individual: a mother, a child, and the child’s alleged father. The autoradiograph compares the DNA bands of each individual. As we have already learned, children get half of their bands from their mother and half from their father. Just as in gel electrophoresis, the smaller pieces of DNA are pushed further down the autoradiograph. These bands differ in length from person to person. For this reason, the bands are used as genetic markers. In this activity, each band is designated by a letter, A through Q. The two letters associated with each segment indicate the individual’s genotype. Instructions Under each column in the autoradiograph, write the letters associated with each individual’s genotype. #1 #2 #3 #4 Child ___________ ___________ ___________ ___________ Mother ___________ ___________ ___________ ___________ Father ___________ ___________ ___________ ___________ 2. Based on this information, which alleged fathers could be biologically related to the child? Problem 5 In this imaginary crime case (A), we are trying to determine which suspect is the