黄瓜枯萎病菌培养滤液对某些保护酶活性和化合物含量影响.pdfVIP

黄瓜枯萎病菌培养滤液 对某些保护酶活性和化合物含量的影响 * 王建明，李蕊倩，王玲平，王莉，徐玉梅，张作刚，郭春绒 （山西农业大学农学院植物病理学系 太谷 030801） 摘要：本实验以黄瓜为材料，测定了黄瓜不同抗病性品种用病原菌毒素培养滤液处理后植株体内的某些保护酶 （超氧化物歧化酶SOD、过氧化物酶 POD和过氧化氢酶 CAT）和某些抗性化合物的动态变化。结果表明：在黄 瓜幼苗期毒素培养滤液处理后，抗病品种根部细胞SOD和 CAT活性大于感病品种，但POD活性低于感病品种。 而抗病品种叶部细胞POD和 CAT活性则小于感病品种，SOD活性在处理前3d小于感病品种，但处理后3d则大 于感病品种。同时，这三种酶的活性均为叶部大于根部。不论在根部，还是叶部，抗病品种处理和对照两条动态 变化曲线的吻合程度均明显高于感病品种。培养滤液处理后，根部和叶部细胞内的可溶性糖相对含量、可溶性蛋 白含量和 Vc含量增加的幅度均为抗病品种大于感病品种，而且前两项含量是叶部大于根部，后一项则是叶部小 于根部。 关键词：黄瓜；枯萎病菌；培养滤液；保护酶；抗性物质 Effect of the Culture Filtrate of Fusarium oxysporum f. sp. cucumerinum on some ProtectiveEnzymeActivityandContentsofChemicalCompounds WANGJian­ming，LIRui­qian，WANGLing­ping*，WANGLi，XUYu­mei，ZHANGZuo­gang， GUOChun­rong (DepartmentofPlantPathologyincollegeofagriculture,ShanxiAgricultureUniversity,Taigu,Shanxi030801) Abstract: With the cucumber as the material, the dynamic changes in activities of some protective enzymes, including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT)and contents of some resistant chemical compounds in different cucumber cultivars were studied after treatment with pathogen­toxin culture filtrate. The results showed that the activities of SOD and CAT in root cells were greater in resistant cultivar than that in the susceptible one at cucumber seedling stage after treatment with toxin culture filtrate. But activity of POD was opposite. The activities of POD and CAT in leaf cells were lower in resistant cultivar than in the susceptible one. Resistant cultivar had lower SOD activity than the susceptible one 3 days before treatment, but greater 3 days after treatment. At the same time, the activities of the three enzymes were greater in leaf cells than that in the rootcells. The extent of accordance in the two dynamic curves between treatment and control