硝酸银对小麦幼穗愈伤组织再生及一些酶活性影响.pdfVIP

Effect of silver nitrate on callus regeneration and activity of some enzymes during tissue culture of wheat immature inflorescence 1, 2 1 1 Xiangru Liao , Lei Tang , Feng Zhang 1 Educational Ministry Key Lab of Industrial Biotechnology, School of Biotechnology, Southern Yangtze University, Wuxi, PRC 214036 liaoxiangru@163.com; 2 Department of Life Sciences, Hebei University, Baoding, PRC 071002 Abstract Effects of silver nitrate on callus regeneration and activity of indole-3-acetic acid oxidase and peroxidase, glutathione S-transferase and reductase, aldehyde oxidase, catalase and peroxidase in cells of immature inflorescence of Triticum aestivum L. variety JI135 were investigated. Immature inflorescences with length between 5-10mm were treated with AgNO3 in MS medium in a period of 10 days. The results indicated that callus regeneration was improved by treatment of wheat immature inflorescence with 16 mg/L AgNO3 for 7 days. The activity of indole-3-acetic acid oxidase and peroxidase was reduced obviously by 16 mg/L AgNO3 four days and six days after inoculation respectively, activity of glutathione transferase ， glutathione reductase ，catalase ，and peroxidase were obviously promoted by AgNO3 4 days after the treatment, But changes in activity of the aldehyde oxidase was not significantly influenced by AgNO3. The mechanism of AgNO3 in improving cell regeneration was discussed. Keywords: AgNO3; catalase; glutathione S-transferase and reductase; indole-3-acetic acid oxidase an