春花胡枝子黄酮大孔树脂纯化工艺的研究及纯化前后抗氧化性变化比较.pdfVIP

12 12 Vol. 12, No. 12 2006 12 Chinese Jo rnal of Experimental Traditional Medical Form lae Dec. , 2006 * 周守标 , 陈乃东, 胡金蓉, 罗 琦, 王贵军, 王春景 ( 安徽师范大学生命科学学院, 重要生物资源 护与利用安徽省重点实验室, 安徽 芜湖 241000) [ ] : AB8 : ; , : 03 mg/ mL; , 20~ 40 , pH= 4~ 5 , 70% ; , pH= 4~ 5, 05~ 10 mL/ min , 70% ~ 90% 05~ 15 mL/ min 012 mg/ mL 7843% 2925% ; 0375 mg/ mL 9849% 6302% :AB8 , [ ] ; AB8 ; ; ; ; [ ] R2863, 2855 [ ] B [ ] 2006) Researches on Purification Technologic Conditions of Lesp edeza d unnii Flavonoids with AB8 Macroporous Resin and Comparision of their Purification Antioxidant Changes before and after Being Purified * ZH OUShoubiao , CHEN Naidong , H UJinrong , L UO Qi, WANG Guij un, WANG Chunj ing (Key Lab. of Biologi al Resour es Conservation and Utilization fo Anhui Provin e , College of Lfi e S ien e , Anhui Normal University , Wuhu 241000, China) [Abstract] Objective: To st dy the conditions and parameters of p rifying total flavonoids from L . dunnii with AB8 macroporo s resin and to compare with antioxidant ability before and after being p rified. Method: Some factors which effected p rification in static and dynamic adsorptions were st died. F rthermore, the antioxidant ability were also determined. Result: The concentration of the sample sol tion was 03 mgmL. In static adsorption, the effects were preferable when temperat re was at 20~ 40 and pH val e was 4~ 5 and sing 70% ethanol as desorption reagent. In dynamic adsorption, pH val e of sample sol tion was 4~ 5 and flowing rate of loading was 05~ 10 mLmin. Using 70% ~ 90% ethanol and 05~ 15 mLmin flow rate, the el ting effect was good. The clearance rates of 012 mgmL refinedf