马铃薯腐烂茎线虫28SrDNA_D2_D3区序列分析.pdfVIP

A CTA PHYTOPATHOLOG ICA SIN ICA 39( 3): 25426 1( 2009) 28S rDNAD2 /D3 1, 2 1 2 1 于海英 , 彭德良 , 胡先奇 , 黄文坤 1 ( , 100193; 2, 650201) : rDNAIT S A ( 900 bp) B ( 1 100 bp) 2, A IT S B 200 bp 2, D 2A D 3B 21 1 28S rDNAD2 /D3 PCR , , 780 bp, DNAM AN 5. 2M EGA4, 2 1 28S rDNAD2 /D3 20 , 97. 2% UPGMA A B , : ; D2 /D3; M o lecu l r clon ing nd sequ en ce s n ly s is o f 28S rDNAD2 /D3 reg io ns o f D ity len chus d es tru cto r o n sw ee t po t to in Ch in 1, 2 1 2 YU H iy ing , PENG D eli ng , HU X i nqi, 1 1 HUANG W enkun ( The S t teK ey L bor tory forB io logy of D ise se nd Insect Pests, In stitute of Pl n t Protection, Ch i nese Ac dem y of A gricultur l Sciences, Beijing 100 193, Ch in ; 2 Key L bor tory of Agricultur l B iodiversity nd Pests Con tro ,l M in istry of Educ tion, Yunn n Agricultur l Un iversity, K unm ing 650201, Ch in ) Ab str ct: Tw o types of rDNAITS ( Type A, 900 bp nd Type B, 1 100 bp) w ere demonstr ted to ex ist in D itylenchu s destructor popul tions on sw eet pot to in Ch in . TheD 2/D 3 reg ions of 28S rDNA from 22 popu l tion s ofD. destructor w ere mp lifiedw ith un ivers l prmi er p irsD 2A nd D 3B. A ll 22 popul tions y ielded one sing le fr gm ent bout 780 bp. Sequence n ly sis nd lignm ent w ere conducted by using the softw re M EGA 4 nd DNAM AN5. 2 nd ll22 sequencesw ere subm itted t theG enB nk. The results show ed th t se quence d ifferences of D 2/D3 reg ion w ere only in few sites, nd th t the smi il rity w s 97. 2%. Tw o types cou ld be distinguished in the phy logenetic tree by using UPGM A m ethod. The evo lution ry rel tionship be tw een wt o types nd the ori