壳聚糖相对分子质量对黄瓜幼苗生长及抗性诱导的作用.pdfVIP

V ol. 30 N o . 2 14 8 Journal of East C hina U niv ers it y of S cience and T ech nology 2004-04 1006-30 80( 2004) 02-014 8-05 1, 2* 1 1 2 张文清 , 　徐　欢 , 　夏　玮, 　张元兴 ( 1. 华东理工大学化学系, 上海200237 ; 2 . 华东理工大学生物反应器工程国家重点实 室) 壳聚糖经 -淀粉酶水解并用乙醇溶液纯化可得到不同相对分子质量的壳聚糖。用重均 分子量为20 ku 和1 ku 的壳聚糖处理黄瓜种子和幼苗可以诱导黄瓜植株产生过氧化物酶和-1, 3-葡聚糖酶。壳聚糖的这种诱导效应对剂量和相对分子质量都有依赖, 并有一定的滞后期。壳聚糖 对黄瓜菌核病的抗病性随喷施次数增加而增加。在对黄瓜种子进行浸种处理时, 重均分子量为20 ku 的壳聚糖诱导效果更为明显; 在对黄瓜叶片喷洒处理时, 1 ku 的壳聚糖的诱导效应更为明显。 壳聚糖; 过氧化物酶; -1, 3-葡聚糖酶; 诱导抗性 S641. 3 A Effect of Molecular Weights of Chitosan on Growth of Cucumber Seedling and Induction of Defense Responses 1, 2* 1 1 2 ZHA N G Wen-qing , X U H uan , X IA Wei , ZHA N G Yuan-x ing ( 1. Dep artment of Chemistry ECU T , hanghai 2002 37, China; 2. tate K ey L aboratory of Bioreactor E ngineering ECU T) Abstract Chit osan hydroly zates w it h diff er ent m olecular w eig ht s released enzym at ical ly w er e show n t o induce plant activ it y . Perox idase and -1, 3-g lu canase w ere over-ex pressed f or def en se respon se w hen . - t hey w er e used t o t reat cu cumber plant s Su ch ef fect s of hydroly zat es w ere dose dep endent and m olecular w eig ht -dep endent , and h ad t o under go a lag ph ase. Wit h t he increase of spraying t imes t he ant i-disease rat io t o clerotinia sclerotiorum increased . T he inducing eff ect s o f chito san w it h m olecular w eig ht of 20 ku w er e m ore obviou s w hen cu cumber seed soaked in chito san so lut ion . Ho w ever, chit osan w it