三甲基硅烷衍生化GCMS 与研究延胡索中水溶性非生物碱类化学成分.docVIP

三甲基硅烷衍生化GC-MS 研究延胡索中水溶性非生物碱类化学成分 刘振华1,2，王如伟2，3Corydalis yanhusuo W.T.Wang）中水溶性非生物碱类化合物。方法 延胡索的80%乙醇提取物经DA201大孔树脂柱吸附，收集水洗部位，该水洗脱部位再经732阳离子树脂交换，其水洗脱组分干燥后进行三甲基硅烷衍生化。采用气相色谱-质谱联用技术检测分析，NIST2005标准谱库结合具体衍生物的MS/MS质谱图进行化合物结构推断。结果 气相色谱-质谱联用方法共检出约50个色谱峰，初步鉴定了其中的16个化合物，均为含有羟基或羧基的大极性化合物。结论 这16个化合物为首次从延胡索中发现, 该研究结果为延胡索的深入开发提供了科学依据。 关键词：延胡索；水溶性非生物碱类化学成分；三甲基硅烷衍生化；GC-MS Application of trimethylsilyl derivatization and Gas Chromatography/Mass Spectrometry for the Identification of the water-soluble, non-alkaloid constituents of Corydalis yanhusuo W.T.Wang Liu Zhen-hua 1,2，Wang Ru-wei2，3，He Hou-hong2，Jiang Xun-zhi2，Ye Jian-feng2，Zeng Su1，Hu Ji-qiang2 (1.College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China；2. Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou 310052, China; 3.College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.) Abstract: Objective To study the water-soluble, non-alkaloid constituents of Corydalis yanhusuo W.T.Wang, such as alcohols, acids, monosaccharides and oligosaccharides, etc.. Methods The 80% alcohol extract of Corydalis yanhusuo W.T.Wang passed through DA201 resin eluted with water, and then the eluate passed though 732# cation exchange resin to collect the water eluate, which containing water-soluble, non-alkaloid constituents was trimethylsilyl derived and detected by Gas Chromatography/Mass Spectrometry. The identification was carried out not only by spectral library match using NIST 2005 library, but also by analysis of the El mass spectrum of the TMS derivatives. Results 16 compounds were tentatively identified from about 50 peaks detected by GC-MS and their structures contain the hydroxyl group or the carboxyl group. Conclusion These 16 compounds were found for the first time in Corydalis yanhusuo W.T.Wang. The results will provide more comprehensive information about Corydalis yanhusuo W.T.Wang for the exploitation and application and its preparations. Key words: Corydalis yanhu