差异显示技术的研究NaHCO3 胁迫下星星草基因表达.pdfVIP

植物学通报 2005, 22 (3): 307~312 Chinese Bulletin of Botany 研究论文 差异显示技术研究NaHCO3 胁迫下 星星草基因表达① ② 王玉成 杨传平 刘桂丰 姜 静 ( 东北林业大学林学院 哈尔滨 150040 ) 摘要 用差异显示技术研究NaHCO 胁迫下星星草(Puccinellia tenuiflora)基因的表达。经Reverse North- 3 ern检测, 获得了7个差异表达的基因片段。其中, 6个为胁迫后诱导表达, 1个为胁迫后抑制表达。序列 同源性分析表明, 胁迫诱导表达的6个基因片段中, 1个与钙依赖性蛋白激酶(CDPKs)基因同源性较高, 其余5个可能为新序列, 胁迫后抑制表达的基因片段与假定的adaptor蛋白基因同源性较高。本研究为 进一步研究星星草的抗盐机理奠定了基础。 关键词 星星草, 差异显示, NaHCO 胁迫 3 Studies of Gene Expression in Puccinellia tenuiflora under NaHCO Stress Using Differential Display Technology 3 WANG Yu-Cheng② YANG Chuan-Ping LIU Gui-Feng JIANG Jing (College of Forestry, Northeast Forestry University, Harbin 150040) Abstract The differential display technique was used to study gene expression of Puccinellia tenuiflora treated with NaHCO . Seven differentially expressed gene segments were obtained 3 through Reverse Northern detection. Among them, six segments were induced and one was inhibited by NaHCO . A homologous analysis revealed that one of the six NaHCO -induced 3 3 gene segments was homologous to the genes encoding calcium-dependent protein kinases （CDPKs ）; the other five might be new sequences. The NaHCO -inhibited gene segment was 3 homologous to a putative adaptor protein-encoding gene. This research provided basic informa- tion for further study of the mechanism on salt resistance in P. tenuiflora. Key words Puccinellia tenuiflora , Differential display, NaHCO stress 3 星星草(Puccinellia tenuiflora )属于禾本