玉米两个功能未知转录因子基因转录后加工.pdfVIP

2009 15 4 ): 483~487 2009-08-25 应用与环境生物学报 ， ( Chin J Appl Environ Biol=ISSN 1006-687X DOI: 10.3724/SP.J.1145.2009.00483 * 玉米两个功能未知转录因子基因的转录后加工 ** 王琦琳 牛向丽 刘永胜 (四川大学生命科学学院生物资源与生态环境教育部重点实验室 成都 610064) 摘 要 ZmBH、ZmWD是玉米中分别具有转录因子bHLH和WD重复蛋白结构的两个功能未知基因，在不同组织中及 各种胁迫诱导条件下，这两个基因均发生不同程度的剪接，产生大小各异的转录本. 序列分析发现其剪接方式与经典 的mRNA剪接不同，即剪接位点的选择不符合 GT-AG或AT-AC规则，而是在剪接位点具有短正向重复序列 SDR (Short- direct repeats). ZmBH 的转录后加工导致序列发生移码而提前终止，而ZmWD则缺失了大部分结构域序列，经由这种方 式产生的转录后加工产物可影响蛋白编码，因而可能是对这两个基因功能进行调控的一种方式. 图6 表 1 参 18 关键词 玉米；转录后加工；转录因子；短正向重复序列 CLC Q78 : S513.01 Post-transcriptional Processing of Two Functions of Unknown Transcription Factor Genes in Zea mays* WANG Qilin, NIU Xiangli LIU Yongsheng** (Key Laboratory for Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China) Abstract ZmBH and ZmWD, containing bHLH or WD40-repeat demain, are unknown function transcription factor-like genes in maize. Under different treatments, various transcripts were determined in the two genes. Sequence analyses demonstrated that unlike the classic splicing processes, the splicing sites were neither GT-AG nor AT-AC, but short-direct repeats (SDRs), which present surrounding splicing junctions in the individual splicing events. The unusual post-transcriptional processing led to a premature termination by introducing stop codon in ZmBH, and a deletion of functional domain in ZmWD. Therefore, the majority o