银屑病皮损中细胞凋亡的原位标记检测与bcl-的表达.x.docxVIP

银屑病皮损中细胞凋亡的原位标记检测与bcl-2的表达银屑病皮损中细胞凋亡的原位标记检测与bcl-2的表达中华皮肤科杂志 1998年第2期第31卷 论著作者：倪晓　孙建方　杨海平　曾学思　桑红桂　李阿梅　高新元　叶干运单位：210042 南京，中国医学科学院　中国协和医科大学皮肤病研究所关键词：银屑病；细胞凋亡；bcl-2【摘要】　目的　探讨细胞凋亡与银屑病发病的关系。方法　用末端脱氧核苷酰转移酶(TdT)介导的d-UTP-生物素缺口末端标记技术(TUNEL)，原位检测了银屑病皮损的凋亡角朊细胞，应用免疫过氧化酶技术研究了正常人皮肤与银屑病皮损的p53蛋白、增殖细胞核抗原(PCNA)和bcl-2的表达。结果银屑病表皮各层中，大量角朊细胞呈现出细胞凋亡的形态学和生化特征。基底层及表皮中下部各层大量角朊细胞PCNA染色强阳性，基底层中bcl-2阳性细胞明显减少。结论　在银屑病皮损中角朊细胞凋亡增加，推测可能是针对细胞过度增殖的一种稳态机理。Detection of Apoptosis by in Situ Labeling and Study on the Expression of bcl-2 in Lesions of Patients with PsoriasisNi Xiao, Sun Jianfang, Yang Haiping, et al. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042【Abstract】　Objective　To investigate the involvement of apoptosis in the lesions of patients with psoriasis. Methods　The apoptosis was detected with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), and the expression of p53, PCNA and apoptosis-suppressing protein bcl-2 was assessed with immunoperoxidase technique in psoriatic lesions and normal skin.Results　A large number of keratinocytes showing biochemical and morphologic features of cells undergoing apoptosis were observed in all the suprabasal layers of the psoriatic epidermis. The plaques from all patients analysed showed marked increase in the number of PCNA positive cells in the middle and basal keratinocytes, and a dramatic reduction in the number of bcl-2 positive cells in the basal cell layer.Conclusion　The increased apoptosis of keratinocytes in the lesions of psoriasis might be a homeostatic mechanism to the hyperplasia of cells.【Key words】　PsoriasisApoptosisbcl-2为了探讨银屑病的发病与细胞凋亡的关系，我们采用末端脱氧核苷酰转移酶(TdT)介导的d-UTP-生物素缺口末端标记(TUNEL)技术，分析了银屑病角朊细胞的凋亡情况，并与常规HE染色进行了对比研究，同时检测了银屑病皮损中p53蛋白、增殖细胞核抗原(PCNA)及凋亡抑制蛋白bcl-2的表达。资料和方法(一)病例：共18例患者，其中14例寻常型患者，男8例，女6例，均为静止期斑块型，2个月内未接受系统及局部治疗；4例红皮病型银屑病，男女各2例。患者年龄15～50岁(平均33.7岁)；病程1个月至30年(平均6.2年)；PASI评分5.8～60.0(平均30.4分)。对照标本10份，均为外科手术正常皮肤。(二)实验方法：皮损边缘钻孔取材，10%中性福尔马林液固定，石蜡