CXCR2基因多态性与奶牛乳房炎和乳品质关联.pdfVIP

HEREDITAS (Beijing) 2008 4 , 30(4): 463―468 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.00463 CXCR2 徐敏, 平富强, 陈仕毅, 赖松家, 刘益平 , 625014 摘要: 采用PCR-SSCP 技术研究了荷斯坦牛、西门塔尔牛和通江黄牛 3 个品种共 160 头个体CXCR2 基因多态 性与乳房炎抗性性状和对牛奶品质性状的遗传效应。结果表明: CXCR2 基因有3 个SNP 多态位点, 分别位于序 列的第685 bp 、777 bp 和861 bp 位点, 确定了5 个等位基因A 、B 、C、E 和F 。685 bp 位点表现为BC 和CC 基因型, 777 bp 位点表现为AA 和AB 基因型, 861 bp 位点为 CC 和BC 基因型。与奶牛乳房炎敏感性有关的基因 型主要是BC 、CC 和FF , 可能对乳房炎有抗性的是AA 、AB 和EE 基因型。据不同基因型对乳品质的遗传效应 分析来看, AA 、AB 和EE 基因型的乳品质性状极显著或显著优于其他基因型。 关键词: CXCR2 基因; 多态性; 奶牛乳房炎; 乳品质 Study on the relationships between polymorphisms of CXCR2 gene and milk quality and mastitis of dairy cow XU Min, PING Fu-Qiang, CHEN Shi-Yi, LAI Song-Jia, LIU Yi-Ping College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China Abstract: PCR-SSCP was applied to analyze the polymorphisms of CXCR2 gene and its relationships with milk quality and mastitis in 160 cattle samples including Holstein dairy cow, Simmental dairy cow and Tongjiang cattle. The results showed that there were 3 SNP loci in CXCR2 gene, which located at 684 bp, 777 bp, 861 bp, respectively. Five alleles (A , B, C, E and F) were determined based on the 3 SNP loci. The genotypes for each locus were BC and CC, AA and AB , CC and BC at 685 bp, 777 bp and 861 bp, respectively. Results also demonstrated that genotypes BC, CC and FF were probably relevant with mastitis, but AA , BB and EE may resist the mastitis of cattle. The genotypes AA , AB and EE may have better milk qual- ity (extremely significant difference or significant difference) than other genotypes. Keywords: CXCR2 gene; polymorphism; mastitis; milk quality CXCR2 G , [4~6] , CXCR2 , Ca2+ , [1,2]