被动吸烟对大鼠卵巢及胚胎发育的影响.pdfVIP

山东医药2010年第5O卷第6期 被动吸烟对大鼠卵巢及胚胎发育的影响 薛德娜 ，李若葆 ’。任春娥 (潍坊医学院，山东潍坊261042) 摘要：目的 观察被动吸烟对大 鼠卵巢功能和胚胎发育的影响。方法 健康雌性Wistar大 鼠8O只，随机分为 对A、B两组。A组 (25只)为正常对照组，B组 (55只)被动吸烟3个月建立大鼠被动吸烟模型。取 A组大鼠14 只、B组 15只心脏取血测E2、P。剩余A组、B组大 鼠雌雄合笼 ，计数怀孕大鼠。B组怀孕大鼠再随机分为B1组和 B2组。B1组停止吸烟，和A组在正常条件下饲养；B2组继续被动吸烟。怀孕20d解剖孕鼠，记录每组胚胎植入 数 ，活胎、吸收胎、死胎、畸胎数及胎重、身长、尾长等。结果 被动吸烟模型建立后，B组大鼠血清中 、P水平与A 组相比明显降低(P均0．05)。雌雄合笼后B组大鼠怀孕率与A组相比明显降低 (P0．01)。B1、B2组大鼠与A 组相比及B2组大鼠与B1组相比吸收胎、死胎率升高，胎鼠体重、身长、尾长降低，P均 0．05。结论 被动吸烟严 重影响雌性大 鼠卵巢功能及胚胎发育。孕后戒烟并不能完全消除被动吸烟对胚胎的影响。 关键词：被动吸烟；大鼠；雌激素；孕激素；胚胎发育 中图分类号：R321．5 文献标志码：A 文章编号：1002-266X(2010)06-0007-03 Effectofpassivesmokingonovarianfunctionandtheembryodevelopmentinrats xUEDe．眦 。U Ruobao．PEN Chun．e (We~ngMedicalCollege，We ng261053，P．R．China) Abstract：0bjec~dve Toobservetheeffectsofpassivesmokingontheovarlanfunctionsandemb~odevelopment． Methods EityWistarfemaleratsweredividedintotwogroupsrandomly：controlgroup(Agroup，25rats)nadexperi— mentalgroup(Bgroup，55rats)．Inthechambermadebyourselvesforpassivesmokingexperiment，theBgroupratswere establishedananimalmodelofpassivesmokingfor3months．Theserum from 14ratsingroupA and15 rats inrgoupB weregained，hteestradiolandpm~stemnelevelweredetectedbyenzymelinkedimmunosorbentassay．Theotherratswere pregnant，thentheprengantratsweresubjectedtodifferentgroups．RatsingroupB1quitedpassivesmoking，ratsingroup B2werestillexposedtotobaccosmoke．Thenthenumberofimplantations，livings，absorbedfetuses，deathsandtherateof malformationwere countedforeachrgoupanddealtwithstatisticalanalysis．Results TheOD ratioofestradiolandproges— teronewerelowerinDrgoupBthna groupA(P0．05)．Therewereobviousdifferenceonabsorbedordeathemb~oBum— hernademb~owei~t，IUGRpercentage，lenghandbetweenhtegroups．Theweightofemb~oinallsmokingrgoupswere obviouslowerthanthatincontrolgroup．Conclusion SecretoryfunctionofovaryWas decreasedbypassivesmoking．Pas— sivesmokingcoulddisturbtheembryodevel