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维普资讯
农业环境科学学报 2007,26(6):2183—2188
JournalofAgro-EnvironmentScience
2,4一D除草剂降解菌的分离及其生物学特性的研究
卫亚红,张晓燕,曲 东
(西北农林科技大学资源环境学院,陕西 杨凌 712100)
摘 要:从陕西省关中地区的农田及菜园土壤中分离筛选出23株能够以2,4-D为惟一碳源的细菌。采用以2,4-D为惟一碳源的
无机盐培养液,在 30℃及 180rm·in。的恒温摇床中对不同菌株进行培养,通过测定培养 12h后培养液中剩余的2,4-D浓度。计算
不同菌株对2,4-D的表观降解率。结果表明,菌株SX09、SX13、SX15和SX21对2,4-D初始浓度为0.5g.L-一时的降解率分别达到
40.93%、38.00%、35.47%和39.02%。降解特性试验表明,选择2,4-D浓度分别为0.14、0.50、1.40和3.00g·L一一条件下,4株细菌降
解 2,4-D的最适浓度为0.50g·L~。4株细菌利用2,4-D生长的最适温度为35℃。菌株SX09、SX13和SX21的最适生长pH值在
7.0—8.5之间,而菌株SX15最适生长pH值为7.0。
关键词:2,4一D:土壤污染:微生物降解
中图分类号:X172 文献标识码 :A 文章编号:1672—2043.(2007)06—2183—06
IsolationofBacteriaforDegradationofHerbicide2,4-DandTheirBiologicalCharacteristics
WEIYa—hong,ZHANGXiao-yan,QUDong
(CollegeofResourcesandEnvironment,NorthwestAFUniversity,Yangling712100,China)
Abstract:Soilcontaminationisabigproblem aroundtheworld.Nowadaysthefarm andvegetablesoilsareseriouslycontaminatedby2 4一
,
dichlorophenoxyaceticacid(2,4-D),whichisextensivelyusedinagricultureasherbicides.Thispaperfocusedontheenrichment.isolation
anddegradationcharacteristicsof2,4-Ddergadationbacteria.2,4一Dusedasasolesourceofcrabonintheculturalmedium,23degradation
bacteriawereisolatedandscreenedfrom thefarm andvegetablesoilsamplesinGuanzhongdistrict,Shaanxiprovince.Aftershakingat30oC
andat180r。min~for12hontheinorganicsaltliquidmedium inwhich2,4-DWas usedas asolesourceofcrabon,theapparentdergading
ratesofthe4selectedstrainsSX09,SX13,SX15na dSX21were40.93%,38.00%,35.47% and39.02% respectively.Th eoptimum concen—
rtafionof2,4-Dforthe4bacteriaWas 0.50g-L~.Basedonthetestinshakingflasks.hte4srtainscangrowbestat35oC.Th eoptimum pH
forstrainsSX09,SX13na dSX21Was 7.0—8.5,na dhteoptimum pH ofrstrainSX15Was 7.0.Th isstudywillprovidetheascendantsrtainsofr
htebiodergadationof2,4-D.
Keywo
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