干热河谷地区几种豆科植物根瘤菌纯培养及生理生化初步与研究论文.doc

西南林业大学 本科毕业（设计）论文 （2010届） 题 目:干热河谷地区几种豆科植物根瘤菌纯培养及生理生化的初步研究 Preliminary study of several legume rhizobia purification and physiological and biochemical characteristics in dry-hot valley 教学院系: 保护生物学学院 专 业: 植物保护06级 学生姓名: 代志浩 指导教师: 张东华（讲师） 评 阅 人: 刘丽（实验师） 2010年 6 月 8日 干热河谷地区几种豆科植物根瘤 菌纯培养及生理生化的初步研究 代志浩 （西南林业大学 保护生物学学院 昆明 650224） 摘要: 本试验对采自云南元谋和元江干热河谷地区的10种豆科植物根瘤样品进行了分离纯化培养，共纯化出34株菌，其中具有根瘤菌特征的的有4个菌株，对其进行耐酸碱性、耐盐性、生长温度范围3种生理生化的初步分析测定，所测4个菌株均表现出耐酸碱性和耐盐性的性状，在短时间的低温和高温下处理后，仍能保持活性，表现出良好的生长势。并对分离出来的10株革兰氏阴性菌进行回接实验，选择沙土、蛭石、营养液等不同的培养载体，并选择台湾相思作为回接豆科植物，以确定供试豆科植物与所分离培养菌种之间的共生结瘤关系、获得并保存高效优良的根瘤菌菌株。 关键词: 干热河谷 根瘤菌 分离纯化 回接试验 生理生化 Preliminary study of several legume rhizobia purification and physiological and biochemical characteristics in dry-hot valley Dai Zhihao (Faculty of conservation biology ,Southwest Forestry University, Kunming,650224) Abstract: This study taken ten kinds of legume rhizobia for purified culture from theYuanmou and Yuanjiang dry-hot valley, and 34 strains were purified, which has the characteristics of Rhizobia strains,4. Determined by the preliminary analysis, 4 strains showed acid and salt tolerance traits, in short low and high temperature treatment, remain active and showing good growth potential. On the isolated 10 strains of Gram-negative bacteria to-back experiments, selected the sand, vermiculite and nutrient solution such as different training vectors, and select Acacia legumes as back then, to determine the tested legumes and The strains were isolated and cultured relations between the symbiotic nodulation, acquire and save the high quality of the Rhizobium stra.Key words: dry-hot valley, rhizobium, purification, inoculating test, physiological and biochemical. 目录 1 研究背景 1 1.1 干热河谷概况 1 1.2 生物固氮 1 1.3 豆科植物的根瘤菌 2 1.4 干热河谷根瘤菌国内外研究进展 3 1.4.1 根瘤菌耐旱性研究进展