过氧化氢诱导的罗非鱼皮胶原纤维降解过程的研究_刘小玲何虹姜元欣江虹锐李全阳.pdfVIP

网络出版时间：2014-01-29 10:50 网络出版地址：/kcms/detail/11.2206.TS1050.042.html 2014-01-27 过氧化氢诱导的罗非鱼皮胶原纤维降解过程的研究（Ⅱ） ——降解过程中产物组成与性质的变化 刘小玲 何虹 姜元欣 江虹锐 李全阳 广西大学轻工与食品工程学院，530004，南宁 摘 要：本文分析了过氧化氢溶液体系中罗非鱼皮胶原纤维热降解过程，反应体系中降解产物的氨基 酸组成、相对分子质量和性质的变化。结果显示，反应 1h 内，溶液释放出的胶原蛋白降解产物极少， 不足以测试样品的凝胶强度和粘度。反应 1~3h,反应体系中溶液的表观粘度增加,反应液冷却后可凝 结成凝胶，凝胶强度随时间逐渐增大。说明释放到水溶液中胶原分子数量增加，溶质浓度提高。反 应 3~5h 后，反应液表观粘度随时间下降，反应液中溶质浓度提高，凝胶的凝胶强度下降，说明这个 阶段以原胶原分子肽链的裂解为主体，导致胶原蛋白进一步水解断裂。电泳结果显示，反应 4h 的样 品，相对分质量在 130kDa 以下的条带比例增加，说明该样品中 α链断裂片段增加。氨基酸组成分析 显示，降解产物与鱼皮氨基酸组成略有差异，但不同反应时间点，明胶产物的氨基酸均没有显著变 化，说明过氧化氢协同热效应，主要是通过水解肽键的方式实现胶原纤维向可溶态明胶的转变。 关键词：罗非鱼；胶原纤维；过氧化氢；降解 Hydrogen peroxide-induced degradation of type I collagen fibers of tilapia skin (I) —changes of composition and properties of collagen extract during degradation Liu xiaoling He hong Jiang yuanxin Li quanyang College of Light Industry and Food, Guangxi University, Nanning, 530004 Abstract: The amino acid composition, molecular weight and properties of extract of collagen degradation induced by heat and hydrogen peroxide were studied. Experimental results show that collagen fibers degradation can be divided into three stages. At the first stage, collagen fiber contracted when heated and there wasn’t significant change in the reaction solution. In the second stage, collagen fibers and microfiber disrupted and procollagen released into solution which made the apparent viscosity of reaction solution increase, which also made reaction solution gelation and gel strength