甲基麝香草酚兰法在尿钙试纸中的应用.docVIP

甲基麝香草酚兰法在尿钙试纸中的应用 北京师大二附中高二年级：翟羽佳 摘要 钙对青少年的成长发育和维持人体正常生理功能极为重要。钙代谢异常将会影响许多器官的生理功能，对于身体健康有着重要影响。因此，建立简便快速、准确性较高、成本低、易普及的钙的检测方法具有重要意义。本研究建立了利用甲基麝香草酚兰分光光度法直接测定尿钙的新方法，并且根据其原理制备了尿钙检测试纸。该方法线性范围为0～120.0mg·L-1，回收率为104.9％～106.4％，相对标准偏差为0.5％，准确度、精密度均较好；尿钙试纸盲测结果与分光光度法比较，符合率为75％，室温保存至少可稳定70天，性质仍然稳定。尿钙试纸使用简便、准确性较高、稳定性好，利于家庭自检及普及。 关键词：尿钙，甲基麝香草酚兰，分光光度法，尿钙试纸 Abstract Calcium is the fifth most abundant element by mass in the human body, where it is a common cellular ionic messenger with many functions, and serves also as a structural element in bone.Calcium metabolism will affect the physiological functions of many organs. As a result, it is important to establish a new method of the determination of calcium, which is simple, rapid, high accuracy and low cost . In this paper, the new method of the determination of calcium which used MTB-Spectrophotometry was established. I also created the urine test paper in accordance with its principle. The linear range of this method is 0 ~ 120.0 mg · L-1, recovery is between 104.9％ and 106.4％The relative standard deviation is 0.5%, accuracy and precision are perfect; comparing with urine test paper blind test results and spectrophotometry , the meeting rate is 75％, and it can be kept stable for at least 70 days at room temperature, remain stable in nature. rine test paper is easy to use, high accuracy, stability, family -friendly self-inspection and penetration. It’s valuable for all of us to use it in daily life. Keywords MTB, spectrophotometry, Urine of Ca2+, urine test paper 1 引言 钙是人体中含量最高的无机盐类物质，也是人体内含量较多的矿物质元素，在体内仅次于氧、碳、氢、氮，居于体内元素第五位。成人体内总钙量约为1300 g，约占人体总重量的2%，正常人体内绝大多数的钙分布于细胞内液，主要以骨盐的形式存在于骨骼和牙齿中，约99%在骨骼，0.5%在牙齿，0.5%在软组织。存在于细胞外液的钙仅占总钙量的0.1%，约1 g左右，0.70 g在血管外液，0.30 g在血浆。钙的排出主要经粪便和尿，机体对粪钙排出的调节能力差，而对尿钙的排出具有一定的调节能力，血钙增多，尿钙排出增多，但很少超过500 mg/天，血钙下降，尿钙排出减少，甚至停排[1-2]。 生命的特征之一就是代谢，钙代谢异常将会影响许多器官的生理功能，对于身体健康非常重要。人体中的钙在神经脉冲传递、触发肌肉收缩、释放激素及调节心率中起着重要作用：缺钙可以引起手足抽搐、肌肉痉挛、喉鸣、惊厥、心律失常，导致骨质钙化障碍，表现为小儿佝偻病、骨骼畸形、发育迟缓，成人骨质软化、骨质疏松等症，对孕妇、婴幼儿影响很大，对中老年人则易造成骨折；钙过量可引发胆结石、肾