PDTC抑制NF-κB的表达对蛛膜下腔出血后脑血管重构中作.docVIP

PDTC抑制NF-κB的表达对蛛网膜下腔出血后脑血管重构中作用的实验研究 张建忠1,2 龙连圣1 辛志成1 李夏良1 蒋超超1 周政3，* 1.解放军第98医院神经外科，浙江湖州 313000 2.第二军医大学长海医院神经外科，上海200433 3.第三军医大学新桥医院神经外科，重庆400037 作者：张建忠,主治医师，Tel：0572-3269875，E-mail：ghost919@126.com *通信作者 (corresponding author)．Tel：023E-mail：bigbrain@ 摘要 目的：探讨应用PDTC抑制核转录因子κB (NF-κB)对兔蛛网膜下腔出血模型基底动脉中的表达及其对脑血管平滑肌增殖的影响。方法：将16只雄性新西兰大白兔随机分为2组，SAH组和PDTC组各8只，枕大池二次穿刺并注入自体动脉血组为SAH组， SAH组应用PDTC注入枕大池作为干预组。模型制作完成后7d处死动物，取其基底动脉测量平滑肌厚度。免疫组化法及Western-blot法检测基底动脉中NF-κB、PCNA蛋白的表达。结果：与SAH组相比， PDTC干预后，基底动脉中NF-κB与PCNA蛋白表达减少，平滑肌厚度变薄，与SAH组比较具有显著差异性(P0.05)。结论：PDTC可通过抑制NF-κB的激活减轻SAH后脑血管壁平滑肌增殖性改变。　 关键词 蛛网膜下腔出血；增殖；核转录因子；吡咯烷二硫氨基甲酸酯 The role of cerebrovascular remodeling in PDTC inhibit the expression of NF-κB after subarachnoid hemorrhage ZHANG Jian-zhong1,2，， ， ， ， 1.Department of Neurosurgery, Peoples Liberation Army 98th Hospital，Huzhou Zhejiang,31300 2.Department of Neurosurgery, Changhai Hospital, Second Military Medical University，Shanghai 200433.Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University，ABSTRACT Objective Aim to investigate the impact of cerebrovascular smooth muscles proliferation to utilize PDTC inhibit the expression of NF-κB rabbit model after subarachnoid hemorrhage(SAH)in the basilar artery. Methods Sixteen New Zealand male rabbits were randomly divided into two group，PDTC group and SAH groups eight each other。And using double injections of fresh autologous arterial blood into the cisterna magna to establish animal models of cerebral vasospasm．Injected PDTC to cisterna magna of S group each day as PDTC group. After injection 7th day,the expression of NF-КB of basilar artery in rabbits were determined by immunohistochemistry and Western blot；morphological changes of rabbit basilar artery was also observered . Results.Compared with SAH group, applied the PDTC intervention, the expression of NF-κB p65and PCNA protein decreased, and smooth muscles thickness became thinner, compared with SAH group has significant differences (p