异养小球藻主要营养成分及氨基酸组成分析_董黎明.pdfVIP

232 2012, Vol. 33, No. 03 食品科学 ※营养卫生 异养小球藻主要营养成分及氨基酸组成分析 董黎明，汪 苹，李金穗，孙 阳 (北京工商大学食品添加剂与配料北京高校工程研究中心，北京市食品风味化学重点实验室，北京 10004 8) 摘 要：测定来自不同产地的4 株蛋白核小球藻和2 株椭圆小球藻异养培养后的主要营养成分和氨基酸组成，对其 营养进行评价，并利用聚类和主成分分析探讨异养小球藻在营养成分和氨基酸含量上的差异。结果显示：异养小 球藻主要营养成分均低于文献报导的自养小球藻，4 株异养蛋白核小球藻和2 株椭圆小球藻必需氨基酸占氨基酸总量 的比例分别为34.94%～37.45% 和37.70%～38.32% ，必需氨基酸指数分别为37.50～49.52 和13 1.89～135.77 。影响 异养蛋白核小球藻营养品质的限制性氨基酸为色氨酸、苏氨酸和异亮氨酸。聚类分析将4 株异养蛋白核小球藻和2 株异养椭圆小球藻各归为一类。主成分分析提取出两个主成分因子，主成分1 和主成分2 的贡献率分别为76.23% 和 17 .80% ，累积贡献率达94 .0 3% 。 关键词：异养小球藻；营养成分；氨基酸；聚类分析；主成分分析 Analysis of Nutritional Components and Amino Acid Composition of Heterotrophic Chlorella sp. DONG Li-ming ，WANG Ping ，LI Jin -sui ，SUN Yang (Beij ing Key Laboratory of Food Flavor Chemistry, Beij ing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beij ing Technology and Business University, Beij ing 100048, China) Abstract：Four strains of Chlorella py renoidosa and 2 strains of Chlorella ellip soidea under heterotrophic culture were analyzed for nutritional components and amino acid composition. Cluster analysis and principle component analysis were conducted to distinguish the differences among the heterotrophic strains of Chlorella sp .. The results showed that the contents of main nutritional components of the heterotrophic strains of Chlorella sp . were lower than previously reported results for autotrophic Chlorella sp .. The ratios of essential to total amino acids of the strains of Chlorella py renoidosa and Chlorella ellip soidea were 34.94% － 37.45% and 37.70% －38.32%, and the essential amino acid index