Ech1的表达下调对小鼠肝癌淋巴道转移的影响及其可能机制.pdfVIP

2015 2 23 4 MODEＲN ONCOLOGY ，Feb. 2015 ，VOL. 23 ，NO. 04 ·437 · 现代肿瘤医学 年 月 第 卷第 期 ?论 著? ?基础研究? Ech1 的表达下调对小鼠肝癌淋巴道转移的影响及其可能机制 1，2 1 1 1，2 1，2 1 1，2 ， ， ， ， ， ， 张 军 金艳玲 毛 俊 魏元怡 黄玉红 王 波 唐建武 The effect of Ech1 downregulation on lymphatic metastasis of mouse heptocarcinoma and the mechanism 1，2 1 1 1，2 1，2 1 1，2 Zhang Jun ，Jin Yanling ，Mao Jun ，Wei Yuanyi ，Huang Yuhong ，Wang Bo ，Tang Jianwu 1 2 Dep artment of Pathology ，Dalian Medical University ，Liaoning Dalian 116044 ，China ;Key Laboratory f or Tumor Metastasis of Liaoning Province ，Liaoning Dalian 116044 ，China． 【Abstract 】 Objective :To observe the lymphatic metastasis of mouse heptocarcinoma when Ech 1 protein was down － regulated in Hca － F cells ，and the expression of Ech 1 protein and the tumor related protein Anxa 7 in 6 FAnxa7 － down cell and FEch1 － down cell． Methods :FEch1 downregulated cell (1 × 10 )were inoculated into the feet pad of 615 mice subcutaneously． On the 28th day of postinoculation ，the mice were euthanized and their lymph nodes were isola- ted ，weighed ，sectioned ，and stained with hematoxylin and eosin． The expression of Ech1 and Anxa7 were observed by Western blot analysis in FAnxa7 － down cell and FEch1 － down cell． Ｒesults :Down － regulation of Ech1 could inhibit the lym- phatic metastasis of Hca － F cells ，and increase expression of the tumor related protein Anxa7 ，while down － regulated Anxa7 could decrease the expression of Ech1． Conclusion :Ech1 is an important factor in the lymphatic metastasis of tumors ，Ec