TGF_1促进人子宫颈癌细胞Siha的侵袭_迁移及其机制研究.pdfVIP

Chinese Journal of Cell Biology 2015, 37(1): 59–65 DOI: 10.11844/cjcb.2015.01.0265 TGF-β1Siha (, 400010) -β1(TGF-β1)Siha 5 ng/mL TGF-β1Siha72 h, CCK-8 TranswellTGF-β1Siha ; Western blotTGF-β1 -2(MMP-2)MMP-9VEGFCFTR P50P65E-cadherinVimentin , 5 ng/mL TGF-β1Siha72 h, Siha , MMP-2MMP-9VEGFCFTRP50P65 Vimentin , E-cadherin, TGF-β1Siha MMP-2MMP-9 VEGF, Siha ; -β1; ; ; Investigating the Migration and Invasion of Human Squamous Cervical Carcinoma Line Siha Stimulated by TGF-β1 and Its Underlying Mechanisms Mao Min, Cai Liuyun, Hu Jianguo, Hu Lina* (Department of Obstetrics and Gynecology, the Second Affi liated Hospital of Chongqing Medical University, Chongqing 400010, China) Abstract This research investigated the infl uence of transforming growth factor-beta1 (TGF-β1) on invasion and metastasis of human squamous cervical carcinoma cell line Siha, and might revealed some possible mechanisms. Cultured human cervical carcinoma cell line, Siha, was stimulated with 5 ng/mL of TGF-β1 for 72 h. The morpholog- ic changes were observed under inverted microscope. Cell proliferation, clone-formation, adhering ability, migration and invasion ability changes were evaluated by CCK-8, colony-forming, cell adherence and Transwell assays, respec- tively. The expressions of matrix metalloproteinase-2 (MMP-2), MMP-9, VEGF, CFTR, P50, P65, E-cadherin and Vimentin were detected by Western blot. We found that after TGF-β1 stimulation, Siha cells converted to an elongat- ed spindle-like shape from a “cobblestone” epithelial structure. The migration and invasion ability of Siha cells were promoted dramatically. The expressions of MMP-2, MMP-9, VEGF, CFTR, P50, P65 and