荧光定量聚合酶链反应技术检测孕妇外周血中游离胎儿DNA可行性研究.pdfVIP

缈 呦 i临床研究孽 荧光定量聚合酶链反应技术检测孕妇外周血中 游离胎儿DNA的可行性研究 贾颐舫吴爱华张爱东杨丹彤邱毅 测中具有较高的灵敏性和特异性，FQ—PCR技术可应用于非创伤性产前诊断。 关键词胎儿DNA荧光定量聚合酶链反应非创伤性产前诊断 in of Fluorescent ChainReactiontheMeasurementofCell— Quantitative Polymerase AppH|cation FreeFetalDNAintheMaternal Aihua，Zhang Circulation缸Yifang，Wu Provincial Scienceand 250002 FamilyPlanning Technologylnst如ute，Jinan Abstract the valueof fluorescent chainreaction Objecave：Toexploreapplicationquantitative polymerase ofcell—freefetalDNAinthematernal sex— t}lemeasurement (FQ—PCR)in ofcell—freefetalDNAinthe of18to25 determiningregionY(SRY)gene maternal(gestationalage was with SRY wasdetectedinthe98．65％ofthe weeks)circulationdetected rQ—PCR．Results：强egene from whohad fetuses．NoneoftheSRY wasdetectedinthe from 148women themale samples gene samples female ofcell—freefetalDNA—SRYinthe 139womenwhohadthe fetuses．Conclusion：The analysis gene and be fornon—invasive maternalcirculationwith is sensitive Carl FQ—PCRhi#dy specific．Itdeveloped prenataldiagnosis．