流式细胞术鉴定超微量免疫细胞样本.pptVIP

There are several different types of white blood cells. They all have many things in common, but are all distinct in form and function. A major distinguishing feature of some leukocytes is the presence of granules; white blood cells are often characterized as granulocytes or agranulocytes: Granulocytes (polymorphonuclear leukocytes): leukocytes characterised by the presence of differently staining granules in their cytoplasm when viewed under light microscopy. These granules are membrane-bound enzymes which primarily act in the digestion of endocytosed particles. There are three types of granulocytes: neutrophils, basophils, and eosinophils, which are named according to their staining properties. Agranulocytes (mononuclear leukocytes): leukocytes characterized by the apparent absence of granules in their cytoplasm. Although the name implies a lack of granules these cells do contain non-specific azurophilic granules, which are lysosomes.[4] The cells include lymphocytes, monocytes, and macrophages. * * * * * * * * * * Presentation title in footer | 00 Month 0000 Presentation title in footer | 00 Month 0000 Presentation title in footer | 00 Month 0000 Presentation title in footer | 00 Month 0000 Presentation title in footer | 00 Month 0000 Presentation title in footer | 00 Month 0000 Presentation title in footer | 00 Month 0000 Presentation title in footer | 00 Month 0000 Presentation title in footer | 00 Month 0000 Presentation title in footer | 00 Month 0000 Presentation title in footer | 00 Month 0000 12月2日 血中的单核细胞 白血球细胞 (leucocyte)：粒细胞和非粒细胞 粒细胞（多形核白细胞）: 中性粒细胞，嗜碱性粒细胞，嗜酸性粒细胞 非粒细胞 （单核白细胞）：淋巴细胞，单核细胞和巨噬细胞 背景介绍 G Koenderman L et al. J Leukoc Biol 2000;68:58-64 背景介绍 纵向小鼠模型（非致死）: 重要性：可用于预测疾病风险的增加，纵向评估病情的进展  需求：观察小鼠模型个体在外界刺激或者药物反应中的长期变化 困难：每次可采集的小鼠血液样本体积非常小 小鼠单核细胞标记(占血液中细胞总数3%) CD11b: 表达在多种细胞表面 （髓样细胞，NK细胞， 活化的T细胞，B细胞 ） CD14: 表面表达量低或检测不到 常用 --由此,需要建立有效的实验方案 建立小鼠纵向模型，需要一种流式细胞仪， 可检测超微量血液样品 同时又需保证重复性，数据准确 更加可行的单核细胞标记，