牛清 2012105011 Handmade Somatic Cell Cloning.pptVIP

单击此处编辑母版标题样式 Handmade Somatic Cell Cloning Niu Qing 2012105011 College of Animal Science and Technology The main content Somatic cell cloned piglets What is handmade somatic cell cloning Impact of the technology Summary Somatic cell cloned piglets A litter of 8 somatic cell cloned piglets was born at 15 min to 23 oclock on the September 15th, this is the first time the cloned pigs was produced by using handmade somatic cell cloning nuclear transfer technology in Nanjing Agricultural University. Mechanism Preparation of somatic cell cloned embryos using handmade somatic cell cloning Transplanted embryos into receptor sows Pregnancy on day 114 Parturition Transgenic pigs are produced by using this technology can provide organ transplantation for human, and at the same time it can provide more convenience for the genetic and breeding pigs. What is handmade somatic cell cloning ? Handmade somatic cell cloning is a nuclear transfer technology, which is an improved zona-free nuclear transfer procedure with manual bisection of oocytes, selection of cytoplasts by Hoechst staining, and two-step fusion of somatic cells from primary granulosa cell cultures with two cytoplasts. Because of its low cost, high efficiency, without expensive instrument equipments, handmade somatic cell cloning technology is becoming a mainstream technology in producing transgenic pigs, cattle, sheep and other animals. In a word, this technology is a major breakthrough. Impact of the technology I think handmade somatic cell cloning technology will advance the development and the industrialization of nuclear transfer in future .And this will provide us with more abundant research materials for human medicine, animal husbandry and basic research. Summary Until now, micromanipulators have been