菌根化翅果油树幼苗生长及叶片光合特性的研究.docVIP

菌根化翅果油树幼苗生长及叶片光合特性的研究 袁丽环 ( 山西师范大学生命科学学院，山西临汾 041004 摘 要 以我国二级濒危保护植物翅果油树(Elaeagnus mollis)为供试植物，通过温室盆栽试验，观察了接种和未接种丛枝菌根菌根真菌对翅果油幼苗叶片光合特性的影响。试验设计分3个组：Glomus mosseae(GM)接种组、Acaulospora delicate（AD）接种组、不接种的对照组（CK）。测定了菌根侵染率、生物量、光响应曲线参数、气体交换参数、叶绿素含量等指标。结果显示，菌根真菌对接种组均有侵染，其中， GM的侵染率最大（92.5%），生态学效应最好；与CK组相比，接种组的生物量均明显提高（p 0.05），其中GM组生物量显著增加，是CK组的2.22倍。接种组光饱和点显著高于CK组，而光补偿点却都明显低于CK组；与CK组相比, 接种组幼苗叶片的净光合速率(Pn)、气孔导(Gs)和蒸腾速率(Tr)均有显著提高, 而胞间CO2浓度(Ci)与气孔限制值(Ls)则变化不明显。接种组的翅果油树幼苗叶片叶绿素a/b的值增高，是CK组的1.480倍，接种组叶绿素a含量都显著大于CK组，而叶绿素b的含量与CK组相比，基本上都表现出负增长效应。研究表明，AM菌根增强了苗木对光的利用效率，更加完全地利用红光促进苗木的生长。 关键词 翅果油树，丛枝菌根， 光合作用，叶绿素 中图分类号：Q939.96 文献标识码：A The study of growth and leaves photosynthetic characteristics of seedlings of Elaeagnus mollis colonized by arbuscular mycorrhizal fungi YUAN Li-Huan （School of Life Sciences, Shanxi Normal University, Linfen, Shanxi 041004, China） Abstract Arbuscular mycorrhizal (AM) fungi were inoculated into the seedlings of Elaeagnus mollis, an endangered plant in China. Our objectives are to study the impact of AM fungi inoculation on the leaves photosynthetic characteristics of E. mollis seedlings using a greenhouse pot experiment. The experimental design included separate inoculation (Glomus mosseae = GM or Acaulospora delicate = AD); control groups were not inoculated. We determined the infection rate of AM fungi, biomass, light response curve parameters, gas exchange parameters and chlorophyll content in the rhizophers. AM fungi infected the inoculated groups, among which the infection rate of GM was the greatest, up to 92.5%. Compared with the control group, the biomass of inoculated groups improved markedly (p 0.05) and the biomass of the GM group was 2.2 times that of the CK group. The light saturation point of the inoculated groups is significantly higher than that of the CK group, but the light compensation point of the inoculated groups is significantly lower than that of CK group. Compared with the CK group, the net photosynthetic rate (Pn), stomat