弓状核损毁对小鼠ghrelin餐前分泌峰的影响_医学论文.docVIP

弓状核损毁对小鼠ghrelin餐前分泌峰的影响_医学论文 弓状核损毁对小鼠ghrelin餐前分泌峰的影响_医学论文 作者：管洪在 李清春 陈曦 蒋正尧 【摘要】 目的 观察损毁新生小白鼠弓状核后是否会引起血浆ghrelin水平的变化。方法 取第12周正常对照小鼠和谷氨酸单钠(MSG)处理的小鼠，内眦取血，酶联免疫分析(EIA)法测血浆ghrelin水平小鼠弓状核冷冻切片，甲酚紫染色观察尼氏体变化取小鼠胃体黏膜组织，Westen blot检测proghrelin蛋白表达的变化。结果 MSG处理组小鼠下丘脑弓状核尼氏体神经元数量明显少于正常对照组小鼠(t=5.21,0.01)。两组小鼠血浆ghrelin水平在禁食前后均有明显变化，但两组小鼠之间比较差异无显著性。与禁食前相比，禁食48 h后两组小鼠胃体黏膜组织中proghrelin蛋白表达水平明显升高，但禁食前后两组小鼠之间比较差异无显著性。结论 下丘脑弓状核对MSG处理的小白鼠血浆ghrelin水平的调控并不是必需的。 【关键词】 弓状核ghrelin小鼠 [ABSTRACT] Objective To explore whether the plasma ghrelin level would be changed due to destruction of arcuate nucleus in newborn mice. Methods Orbital blood was taken from 12weekold and monosodium glutamatetreated (MSG) mice. Plasma level of ghrelin was determined with enzymelinked immune assay (EIA), frozen section of arcuate nucleus with cresyl violet staining used for observing changes of Nissl neurons and Western blot employed to study proghrelin protein expression in the mucosa of mouse gastric body. Results The number of Nissel neuron in arcuate nucleus was significantly reduced in MSGtreated mice compared with that of normal controls (t=5.21,0.01). There were significant changes of plasma ghrelin level before and after fasting in both groups, but the difference was not statistically different. The level of proghrelin protein expression in gastric mucosa was significantly increased after fasting for 48 h in both groups, but that was not statistically different between the two groups.Conclusion The results suggest that typothalamic arcuate nucleus does not play a critical role in regulating plasma ghrelin levels in MSGtreated mice. 　　[KEY WORDS] arcuate nucleus ghrelin mice 　　ghrelin 是由胃黏膜X/A内分泌细胞释放的一种“生长素促分泌素(GHS)”，是GHS受体的内源性配体[1]。最初观察到ghrelin具有强烈刺激生长素分泌的作用，随后很快发现它可刺激摄食和新陈代谢[2]。包括人类在内的多种动物给予ghrelin后均增加其摄食量。ghrelin为迄今惟一的由胃肠道分泌的饥饿激素，是启动摄食的饥饿信号。无论是人还是其他动物，血浆ghrelin都有一个餐前分泌峰[3]，而进食后出现一个餐后抑制。另外，许多文献报道新生鼠给予谷氨酸单钠(MSG)处理可选择性地损毁弓状核NPY/ AgRP神经元[4]，LUQUET等[5]报道弓状核NPY/ AgRP神经元为成年鼠所必须，而新生鼠弓状核损毁后摄食正常。本研究旨在观察损毁新生小鼠弓