应激状态下大鼠肠屏障功能损伤过程中MMP13、LN的改变及意义_医学论文.docVIP

应激状态下大鼠肠屏障功能损伤过程中MMP13、LN的改变及意义_医学论文 应激状态下大鼠肠屏障功能损伤过程中MMP13、LN的改变及意义_医学论文 【摘要】 目的 探讨应激状态下大鼠肠黏膜基质金属蛋白酶（MMP）和层粘连蛋白（LN）的改变及意义。方法 Wistar 大白鼠60只，随机分为3组，每组20只。实验组制备肠缺血再灌注模型，对照组只进行同样腹部手术操作但不夹闭肠系膜上动脉，正常组不手术。6 h后处死大鼠，取距回盲部15 cm处肠管1 cm，常规组织切片，苏木精伊红染色观察肠黏膜组织细胞学形态；用免疫组织化学方法染色显示肠黏膜组织或细胞中LN、MMP的表达情况，并用VIDAS图像分析系统进行半定量分析。结果 苏木精伊红染色显示：实验组与对照组相比，肠壁变薄，上皮细胞水肿明显，部分坏死脱落，中性粒细胞及淋巴细胞浸润。免疫组织化学染色显示：实验组肠黏膜组织中MMP表达较对照组增高，基底膜LN表达较对照组降低，差异有显著性（t=3.31、3.46，0.01），而对照组与正常组相比差异无显著性（Pgt0.05）。结论 缺血再灌注可使肠黏膜组织中MMP水平表达增高，进而使其降解底物LN水平降低，肠黏膜萎缩及通透性增大，导致肠屏障功能损伤。 【关键词】 再灌注损伤 肠屏障 层粘连蛋白 基质金属蛋白酶类 ［ABSTRACT］ObjectiveTo investigate changes of expressions of LN and MMPcontrol. For experimental group, a model of postischemicreperfusion intestine was established by clamping the superior mesenteric artery for control group, a laparotomy was merely done without clamping any arteries for those in normal control group, received nothing. A 1cmlong segment of intestine, 15 cm above the ileocecal junction, was dissected 6 hours later, and a tissue section made as routine. Immunohistochemical study was used to observe the expressions of MMP13 and LN and VIDAS image analysis system applied for semiquantitative detection.ResultsThe thinner enteric wall, edematous epithelium with neutrophils and lymphocytes infiltration, shorter intestinal villi were obviously noticed in the experimental group compared with the other two. Immunohistochemically, the expression of MMP13 in the experimental was higher and the expression of LN was lower compared with those in the control (t=3.31，3.460.01）.ConclusionPostischemic reperfusion may elevate the expression of MMPd leads to lower expression of LN, shrink the mucosa and increase its permeability, thus damage the intestinal mucosal barrier. ［KEY WORDS］reperfusion injury intestinal mucosal barrier laminin matrix metalloproteinases 正常情况下，肠屏障是指肠道能防止肠腔内的有害物质如细菌和毒素穿过肠黏膜进入体内其他组织器官和血液循环的结构和功能的总和。机械屏障是肠道屏障的重要组成部分[1],同时也是诸如免疫屏障、生物屏障等肠道屏障其他组成部分的物质基础，是肠屏障功能维持的关键。机械屏障由肠道黏膜上皮细胞、细胞间紧密连接等构成[2]