应用RTPCR分析食管癌组织中lrig1基因的表达_医学论文.docVIP

应用RTPCR分析食管癌组织中lrig1基因的表达_医学论文 应用RTPCR分析食管癌组织中lrig1基因的表达_医学论文 作者：闫尧　马军　唐芙爱　达嘎　祁秋干　李印 【摘要】 目的: 观察lrig1基因在食管鳞癌中的表达及意义. 方法: 采用RT检测36例食管鳞状细胞癌癌组织、相应的癌旁组织和远癌组织中lrig1 mRNA的表达情况. PCR产物经凝胶电泳，比较3种组织中lrig1与GAPDH条带的灰度值之比，半定量分析lrig1 mRNA的表达水平. 结果: 36例食管癌组织中有15例(42%)lrig1 mRNA检测到阳性表达，21例(58%)lrig1 mRNA表达缺失，其阳性表达率低于相应的癌旁组织（92%）和远癌组织(100.0%,0.05)；癌组织中lrig1 mRNA表达水平(0.76±0.22)低于相应的癌旁组织(0.89±0.33)和远癌组织(1.13±0.40)；随着肿瘤分化程度的升高，lrig1 mRNA在癌组织中的阳性表达率也增加(0.05)，但lrig1 mRNA表达缺失与食管癌临床分期(TNM),淋巴结有无转移和病理类型均无统计学差异(Pgt0.05). 结论: lrig1 mRNA在食管癌组织中存在表达缺失和低表达，提示lrig1基因在食管癌的发生发展中可能具有抑癌基因的作用. 【关键词】 lrig1基因；食管肿瘤；mRNA；逆转录聚合酶链反应 　　【Abstract】 AIM: To study the expression of lrig1 gene in esophageal squamous cancer and its significance. METHODS: The expression of lrig1 gene was detected by RTPCR in cancer tissues, matched paracancer tissues and tissues far away from tumor in 36 cases of esophageal squamous cancer. PCR products were tested by gel electrophoresis.The expressions of lrig1 gene in the three kinds of tissues were semiquantified by calculating the ratio of the gray value of lrig1 bands and those of GAPDH bands respectively. RESULTS: The positive expression rate of lrig1 mRNA in 36 cases of esophageal carcinoma was 41.7% (15/36). The positive rate was lower than that in tumoradjacent tissues (91.7%) or esophageal mucosa far away from tumor(100.0%) respectively (0.05). Semiquantified analysis showed that the level of lrig1 mRNA expression in esophageal cancer tissues (0.76±0.22) was lower than that in tumoradjacent tissues (0.89±0.33) and esophageal mucosa far away from tumor (1.13±0.40). The higher positive expression rate of lrig1 mRNA was correlated with higher differentiation grades in cancerous tissues (0.05). While the expression of lrig1 mRNA had no relationship with TNM stages, lymph node metastasis or histologic types in esophageal cancer (Pgt0.05). CONCLUSION: The expression of lrig1 mRNA is lost or reduced in esophageal cancer tissues, which indicates that the lrig1 gene may