急性白血病患者氧化应激状态与抑郁情绪之间的关系_药学论文.docVIP

急性白血病患者氧化应激状态与抑郁情绪之间的关系_药学论文 急性白血病患者氧化应激状态与抑郁情绪之间的关系_药学论文 作者：周芙玲，张王刚，苏智祥，蒙昕，田纬【摘要】 本研究探讨急性白血病患者氧化应激状态与抑郁情绪之间的关系。用抑郁自评量表（self-rating depression scale， SDS）等调查表对92例初发急性白血病患者的抑郁症状进行评定。检测血浆总抗氧化能力（T-AOC）、活性氧(ROS)、超氧化物歧化酶（SOD）、丙二醛(MDA)及一氧化氮（NO）含量。采用实时定量PCR检测DNA修复基因人8- 羟基鸟嘌呤糖苷酶（human 8-hydroxyguanine glycosylase，hOGG1）的表达。结果证明，急性白血病患者抗氧化酶防御能力下降，抑郁情绪发生率47.83%，伴抑郁障碍的患者T-AOC和SOD水平明显降低，ROS、NO及MDA水平明显升高，DNA修复基因hOGG1 mRNA的表达升高。急性白血病患者病程、hOGG1水平与抑郁程度呈正相关，T-AOC水平与抑郁程度呈负相关。结论： 急性白血病患者存在氧化损伤，伴抑郁症状的患者抗氧化酶防御能力进一步减弱,提示氧化应激与急性白血病抑郁症状的发生和发展密切相关。 【关键词】 氧化应激；急性白血病；抑郁；DNA修复基因hOGG1 　　Relationship between Oxidative Stress and Depression in Patients with Acute Leukemia 　　AbstractThis study was aimed to investigate the relationships between oxidative stress and depression in patients with acute leukemia. Ninety two cases of acute leukemia were randomly enrolled in the study. Depressive disorder was assessed by self-rating depression scales (SDS) and multiple items questionaires. The total anti-oxidation capability (T-AOC), reactive oxygen species (ROS) and superoxide dismutase (SOD) activities, as well as malondialdehyde (MDA) and nitric oxide (NO) levels were measured in pre-treatment periods. Meanwhile, the steady state level of human 8-hydroxyguanine glycosylase（hOGG1）mRNA transcript was monitored by quantitative real-time PCR. The results showed that the defence of antioxidant system was impaired in patients with acute leukemia. The incidence of depression was 47.83% in 92 cases. T-AOC and SOD activities were significantly decreased in patients with depression，while ROS, NO, MDA levels and hOGG1 mRNA expression were reverse of the former. It revealed that depression positively correlated with course of disease and hOGG1, and negatively correlated with T-AOC. It is concluded that oxidative damage occurs in patients with acute leukemia, moreover, lower antioxidant defences exist in depressive patients. These results underscore the notion that oxidative stress may promote the development of d