心理性应激状态下针刺对大鼠DMV放电、胃电和胃粘膜损伤的影响_医学论文.docVIP

心理性应激状态下针刺对大鼠DMV放电、胃电和胃粘膜损伤的影响_医学论文 心理性应激状态下针刺对大鼠DMV放电、胃电和胃粘膜损伤的影响_医学论文 作者：王景杰，黄裕新，郭庆东，秦 明，高 巍，王庆莉 【关键词】 电针 　　关键词: 电针防治作用应激，心理性胃粘膜/损伤 　　摘 要:目的 探讨针刺对心理性应激状态下大鼠胃动力障碍及胃粘膜损伤的防治作用. 方法 雄性SD大鼠96只随机分成3组.A:正常对照组B:心理性应激组C:心理性应激+针刺组.采用高压恒流脉冲刺激器制备心理性应激模型.电针刺激大鼠双侧足三里，细胞外引导测定迷走神经背运动核（DMV）自发放电，浆膜法测定胃电数据及用Chen等法测定胃粘膜损伤指数. 结果 A组大鼠迷走神经背运动核自发放电较少，胃电活动较为规律，胃粘膜组织正常.B组迷走神经背运动核自发放电不规律，以放电频率增加占优势，胃电活动出现紊乱，但与心理性应激的时间和程度无明显相关性，胃粘膜可以出现损害、甚至产生溃疡.且随着受到心理性应激反应程度的不断增强，胃粘膜损害的程度不断加大，其损伤指数逐渐增加.但总体上来看仍B组与C组相比，在心理性应激的2，4，6和8d二者胃电的频率、波幅都有明显的差异（0.05）.相同情况下，C组与B组的胃电差异显著（0.05）然而，C组与A组相比，在心理性应激反应的2，4，6和8d其胃电的频率和波幅无差异（Pgt0.05）.而在心理性应激反应后给予电针刺激的条件下，胃粘膜损伤明显减轻，且随着时间的延长，这种效果更加明显.C组与B组相比，二者的损伤指数有明显差异（0.01）同时，随着心理性应激与给予电针刺激的不断增加，其保护率也在不断的增高. 结论 心理性应激可以导致胃动力障碍和胃粘膜损伤，且关系密切.针刺足三里对其有明显的防治作用. 　　Keywords:electroacupunctureprotective and therapeuticstress，psychologicalgastric motor disordersgastric mucosa/injuries 　　Abstract:AIM To test the protective and therapeutic ef-fects of electroacupuncture on gastric motor disorders and a-cute gastric mucosal lesions under psychological stress in rats.METHODS Ninety-six male Sprague-Dauley rats were randomly divided into three groups:control group（CG，n=32），psychological stress group（PSG，n=32）and elec-troacupuncture group（EAG，n=32）.Every group was ran-domly divided into four subgroups（CG，n=32，n=8PSG，n=32，n=8EAG，n=32，n=8）.Animal model was con-structed with impulsive stimulator of high voltage and stable currency stimulating the Chusanli with electroacupuncture.Then spontaneous electroactivity of dorsal motor nucleus of the vagus nerve（DMV）was recorded extracellularly and EGG with the serosa method，and the gastric mucosa injury index was calculated.RESULTS The spontaneous elec-troactivity from DMV was rarely observed in normal rats.EGG was regular with frequency3per minute and amplitude360～370μV.Gastric mucosa was normal.Under psycholog-ical stress，spontaneous electroactivity from DMV was irreg-ular which did not significantly correlate with time and degree of psychologi