急性脑梗死病人血清sICAM-1与sE-selectin含量变化_医学论文.docVIP

急性脑梗死病人血清sICAM-1与sE-selectin含量变化_医学论文 急性脑梗死病人血清sICAM-1与sE-selectin含量变化_医学论文 ［摘要］目的 探讨急性脑梗死病人血清可溶性细胞间黏附分子-1（sICAM-1）和可溶性E-选择素（sE-selec-tin）水平的变化。方法 采用双抗体夹心酶联免疫吸附法（ELASA）测定100例急性脑梗死病人发病第1、3、5、10天和30例健康对照者的血清sICAM-1、sE-selectin的水平。结果 急性脑梗死病人血清sICAM-1、sE-selectin的浓度在发病第1天即升高，第3天达到高峰，第5天开始下降，第10天显著下降，各时间点水平均高于对照组（ F= 46.63、42.52，t=5.07～18.07，P lt0.01）大梗死灶病人组血清sICAM-1、sE-selectin水平高于中梗死灶病人组和小梗死灶病人组（ F=56.23、47.64，t=4.74～9.26，P lt0.01）。结论 急性脑梗死诱导血清sICAM-1、sE-selectin水平升高，随病程动态变化，且水平与脑梗死体积密切相关。 　　［关键词］ 脑梗死细胞间黏附分子E-选择素 　　［ABSTRACT］ObjectiveTo investigate the dynamic changes of soluble intercellular adhesion molecule-1 （sICAM-1） and soluble E-selectin （sE-selectin） in acute cerebral infarction. MethodsThe levels of serum sICAM-1 and sE-selectin in 100 cases of acute cerebral infarction on the 1st ， 3rd ， 5th ， and 10th day after the onset and 30 control cases were measured separately using two-layer antibody sandwich enzyme-linked immunosorbent assay （ELISA）. ResultsThe levels of serum sICAM-1 and sE-selec-tin in the patients increased significantly on the 1st day， and reached peak until the 3rd day， followed by obvious reduction after five and 10 days. The levels of sICAM-1 and sE-selectin at all time-point increased significantly compared with the control cases （ F=46.63，42.52t=5.07-18.07P lt0.01）. Larger cerebral infarction induced higher level of serum sICAM-1 and sE-selectin than the medium and small cerebral infarction （ F=56.23，47.64t=4.74-9.26P lt0.01）. ConclusionAcute cerebral infarction increases the levels of serum sICAM-1 and sE-selectin， which is changed with the course of the disease and closely associated with volume of the infarcted focus. 　　［KEY WORDS］cerebral infarction intercellular adhesion molecule E-selectin 　　脑梗死是中老年人常见病之一，有较高的致死率及致残率。近年来脑梗死病人半暗区神经细胞损伤与炎症的关系愈来愈受到人们的关注［1］，脑缺血受损部位有白细胞浸润和炎性细胞因子的存在，证明了炎症反应参与了急性脑缺血的神经细胞损伤过程［2］，黏附分子在此过程中起了重要作用。本文观察了急性脑梗死病人血清可溶性细胞间黏附分子-1（sICAM-1）和可溶性E-选择素（sE-selectin）水平的变化，现报告如下。 　　1 资料与方法 　　1.1 对象及分组 选择2003年1月～2005年1月青岛市中心医