慢性前列腺炎病人前列腺液中IL1β、IL8、TNFα和PGE2的变化及意义_医学论文.docVIP

慢性前列腺炎病人前列腺液中IL、IL、TNF和PGE2的变化及意义_医学论文 慢性前列腺炎病人前列腺液中IL、IL、TNF和PGE2的变化及意义_医学论文 【摘要】 目的 探讨慢性前列腺炎病人前列腺液中细胞因子IL、IL、TNF和PGE2的变化与慢性前列腺炎类型、发病机制、症状和前列腺液白细胞计数的关系。方法 以Meares法对100例前列腺炎病人行尿液、前列腺按摩液细菌培养，结合前列腺液常规检查和NIH评分,将其分为慢性细菌组(Ⅱ型，11例)、慢性非细菌组(Ⅲa型，66例)、前列腺痛组(Ⅲb型，23例)。应用放射免疫法测定各组病人前列腺液中IL、IL、TNF和PGE2含量,并与20例正常人进行对照。结果 Ⅱ型前列腺炎病人前列腺液中IL、IL、TNF、PGE2水平明显高于Ⅲa型、Ⅲb型及对照组，Ⅲa型明显高于Ⅲb型及对照组,差异有显著性(F=27.001～59.541，q=3.70～4.50，0.01)；Ⅲb型与对照组相比无明显差异(Pgt0.05)。前列腺炎病人前列腺液IL水平与TNF、PGE2水平呈正相关(r=0.563、0.826，0.01)，白细胞计数与IL水平呈正相关(r=0.547，0.01)，IL、PGE2水平与NIH疼痛不适症状评分呈正相关(r=0.506,0.01)。结论 慢性前列腺炎病人前列腺液IL、IL、TNF、PGE2表达增高可作为慢性前列腺炎的诊断依据之一，对慢性前列腺炎分型可能有一定临床意义。 【关键词】 前列腺炎 白细胞介素1β 白细胞介素8 肿瘤坏死因子α 前列腺素E2 放射免疫测定 ［ABSTRACT］ObjectiveTo study the levels of interleukinleukin8 (IL8), tumor necrosis factorα (TNFα), and prostaglandin E2 (PGE2) in expressed prostatic secretion (EPS) of patients with chronic prostatitis (CP) and their relation with the type, pathogenesis and diagnosis of CP.MethodsIL1β,IL8,TNFα and PGE2 levels were measured in EPS of 100 CP patients and 20 normal controls by radioimmunoassay. Urine and EPS were cultured according to MearesStamey methods. NIHChronic prostatitis symptom index (NIHCPSI) score was done in all patients. The patients were divided into three types, i.e. type Ⅱ(n=11), type Ⅲa (n=66), and typeⅢb (n=23), according to the urine and EPS culture as well as NIHCPSI score.ResultsThe levels of IL1β, IL8,TNFα, and PGE2 in typeⅡgroup were higher than that in typeⅢa, typeⅢb and control groups (q=3.70,4.20,4.500.01), those of typeⅢa group were higher than in typeⅢb group and control group (q=2.80,0.05). No statistically significant difference between typeⅢb group and control group was noted (Pgt0.05). A positive correlation existed between the levels of IL1β and TNFα or PGE2 (r=0.563,0.8260.05). WBC count in EPS showed a positive correlation with IL8 level (r=0.547,0.01). The levels of IL1β and PGE2 were highly related to the NIHCPSI score of pain and/or discomfort (r