慢性哮喘小鼠EGFR的表达及地塞米松对其的影响_医学论文.docVIP

慢性哮喘小鼠EGFR的表达及地塞米松对其的影响_医学论文 慢性哮喘小鼠EGFR的表达及地塞米松对其的影响_医学论文 【关键词】 哮喘 　　Expression of epidermal growth factor receptor and its ligands in chronic asthma mice and the influence of dexamethasone 　　【Abstract】 AIM: To investigate the effect of epidermal growth factor receptor (EGFR) system on the asthmatic airway remodeling.METHODS: The chronic asthma mice model was established by ovalbumin (OVA) immunization. Mice were injected intraperitoneally with dexamethasone of 2 mg/kg 1 h before the sensitization and sacrificed by cervical dislocation 24 h after the last challenge. Conventional pathological slides were also observed to study the pulmonary inflammation and the thickness of airway wall and airway smooth muscles. The concentration of TGFα in bronchoalveolar lavage fluid (BALF) was detected by ELISA. RTPCR was conducted to detect the mRNA expression of EGFR in the lungs. Pulmonary EGFR protein was observed by immunohistochemical staining and Western blot analysis. RESULTS: In chronic asthma mice, inflammatory cell infiltration around bronchi and vessels could been seen on slides. The airway wall and the airway smooth muscles thickened and TGFα in BALF increased. The expression of EGFR mRNA and protein in the lungs increased (0.01). Dexamethasone injection before sensitization decreased TGFα in BALF and the expression of phosphoEGF receptor protein in the lung alleviated the pulmonary inflammation and inhibited the thickening of airway wall and airway smooth muscle (0.01). CONCLUSION: Airway remodeling occurs in the chronic mice asthma model. Early dexamethasone injection can inhibit the expression and activation of EGFR system, decrease the TGFα concentration in BALF and prevent the airway remodeling. 　　【Keywords】 asthma airway remodeling receptor, epidermal growth factor transforming growth factor alpha dexamethasone 　　【摘要】 目的： 探讨表皮生长因子受体（EGFR）在慢性哮喘气道重建中的作用. 方法： 建立小鼠慢性哮喘模型，致敏前1 h气道内滴入地塞米松2 mg/kg，最后一次激发24 h后处死动物，常规病理切片观察小鼠肺内炎症情况、气管壁厚度及气道平滑肌厚度，ELISA法测定肺泡灌洗液中TGFα的浓度，RTPC