手足口病患儿血清IL-6-IL-10-IL-17水平的变化及其临床意义_医学论文.docVIP

手足口病患儿血清IL-6-IL-10-IL-17水平的变化及其临床意义_医学论文 手足口病患儿血清IL-6?IL-10?IL-17水平的变化及其临床意义_医学论文 黄小霏 檀卫平 江润昌 【摘要】 【目的】 探讨手足口病(HFMD)患儿外周血白介素-6(IL-6)?白介素-10(IL-10)?白介素-17(IL-17)水平及其在HFMD发展过程中的变化?【方法】 本实验采用酶联免疫吸附实验(ELISA)双抗体夹心法测定19例HFMD患儿(急性期?恢复期)及同期20例健康体检儿童血清IL-6?IL-10?IL-17的含量并对HFMD患儿急性期?恢复期及健康儿童进行两两比较,了解IL-6?IL-10?IL-17在HFMD发展过程中的变化?【结果】 1?手足口病患儿急性期IL-6? IL-10及IL-17水平显著高于健康对照组(P lt 0.01)恢复期IL-6及IL-17水平与对照组比较无显著差异(P gt 0.05)?而IL-10水平仍高于健康对照组(P lt 0.01)?2?急性期IL-6与IL-10呈负相关关系(P lt 0.05)IL-6与IL-17呈正相关关系(P lt 0.01)IL-10与IL-17呈负相关关系(P lt 0.05)?恢复期:IL-6与IL-10呈负相关关系(P lt 0.05)IL-6与IL-17呈正相关关系(P lt 0.01)IL-10与IL-17无相关关系(P gt 0.05)?【结论】 手足口病患儿急性期炎症细胞因子(IL-6?IL-17)水平显著升高,而抑制性细胞因子IL-10水平升高持续到恢复期? 【关键词】 手足口病 儿童 白介素 　　Abstract: 【Objective】 To study the changes and clinical significance of serum interleukin (IL)-6, IL-10, and IL-17 levels in the children with hand foot and mouth disease (HFMD). 【Method】 The serum concentration of IL-6, IL-10, and IL-17 in 19 cases of HFMD children ( acute stage and convalescence ) and 20 cases of healthy controls were measured by ELISA. The cytokines levels were compared. 【Results】 1.Compared with healthy controls, the levels of IL-6, IL-10, and IL-17 in acute stage of HFMD were significantly increased in HFMD children (P lt 0.01). There was no significant difference of the levels of IL-6 and IL-17 between the convalescence of HFMD and healthy children (P gt 0.05), However the levels of IL-10 in convalescence of HFMD were higher than healthy children (P lt 0.01). 2. Spearman correlation analysis revealed that IL-6 and IL-17 were negatively correlated with IL-10 (P lt 0.05) IL-6 positively correlated with and IL-17 in acute stage (P lt 0.01). In convalescence IL-6 was negatively correlated with IL-10 (P lt 0.05), positively correlated with IL-17 (P lt 0.01) IL-10 and IL-17 have no correlative relation (P gt 0.05). 【Conclusions】 1.The levels of IL-6 and IL-17 were significantly increased in acute stage of HFMD children. 2. The persistent elevation of serum IL-10