抵当芪桂汤对链脲佐菌素诱导糖尿病大鼠血清内皮素-1和一氧化氮的影响_药学论文.docVIP

抵当芪桂汤对链脲佐菌素诱导糖尿病大鼠血清内皮素-1和一氧化氮的影响_药学论文 抵当芪桂汤对链脲佐菌素诱导糖尿病大鼠血清内皮素-1和一氧化氮的影响_药学论文 作者：赵天才 杨景锋 李鹏 袁志敏 张瑛 李月红 张瀚元 【摘要】 目的观察抵当芪桂汤对链脲佐菌素(STZ)诱导糖尿病大鼠血清内皮素-1(ET-1)和一氧化氮(NO)的影响，探讨其防治糖尿病血管病变的作用机制。方法90只SD大鼠随机抽取10只为空白组(A，n=10)，其余大鼠予以链脲佐菌素(STZ)60 mg/kg一次性腹腔注射建立糖尿病大鼠模型。造模成功后，将模型动物随机分为模型组(B，n=13)、达美康组(C，n=13)、抵当芪桂汤组(D，n=13)、抵当芪桂汤加达美康组(E，n=13)、抵当芪桂汤大剂量组(F，n=13)，分别给予相应干预。于灌胃第8周末，分别用ELISA和硝酸还原酶法测定大鼠血清中ET-1和NO含量。结果干预结束后，与B组比较，各干预组空腹血糖(FBG)均降低，其中以C组最为明显(0.05);与B组比较，各干预组血清ET-1均降低、NO均升高，其中以D组最为明显(0.05);与C组比较，D组降低血清ET-1、升高NO疗效明显(0.05)。结论抵当芪桂汤可能通过降低STZ大鼠血清中ET-1含量、升高NO含量，保护血管内皮功能，阻止或延缓糖尿病血管病变的发生和发展。 【关键词】 抵当芪桂汤; 糖尿病血管病变; 内皮素-1; 一氧化氮 　　Abstract:ObjectiveTo observe the effects of Didang Qigui Decoction on endothelin-1(ET-1) and nitric oxide(NO) in serum of diabetic rats, and to further explore its mechanism of prevention and treatment of diabetic angiopathy. MethodsThe model of diabetes was established by intraperitoneal injection of Streptozotocin (STZ) at a dose of 60mg/kg. Experimental rats were randomly divided into six groups: blank group(A, 10 cases), model group(B, 13), diamicron group(C,13), Didang Qiqui Decoction group(D, 13), Didang Qigui Decoction and diamicron group(E, 13), Didang Qigui Decoction high-dose group(F, 13) and they were treated respectively. Eight weeks later, serum ET-1 and NO contents were determined through ELISA and nitrate reductase method respectively. Results (1)After treatment, FBG in each treatment group was decreased dramatically, especially in C group(0.05) compared with B group; (2) In each treatment group, ET-1 was decreased while NO was increased, especially in D group(0.05) compared with B and C group. ConclusionIt is possible that Didang Qigui Decoction protect vascular endothelial cells by reducing serum ET-1 content and increasing NO content and further prevent or delay the occurrence and development of diabetic angiopathy. 　　Key words:Didang Qigui Decoction; Diabetic angiopathy; Endothelin-1; Nitric oxide 　　糖尿病(diabetes mellitus, DM)是由于胰岛素分泌和(或)作用缺陷所引