支气管上皮细胞和嗜酸粒细胞的不同培养方式对IL_医学论文.docVIP

支气管上皮细胞和嗜酸粒细胞的不同培养方式对IL_医学论文 支气管上皮细胞和嗜酸粒细胞的不同培养方式对IL_医学论文 作者：王成彬，黄振国，叶伟基，李洛谊，鲍依稀，童红莉，田亚平，林伟基 【关键词】 支气管；上皮细胞；嗜酸细胞；白细胞介素 【Abstract】 AIM: To investigate the effect of different culture systems on IL6 release in model of coculture of human bronchial epithelial cells and eosinophils. METHODS: Human bronchial epithelial cells and eosinophils were cultured or cocultured in separation, contact and Matrigel system. IL6 release in different culture systems of bronchial epithelial cells and eosinophils was determined by enzymelinked immunosorbent assay (ELISA). RESULTS: Compared with the sum of IL6 in culture supernatants of bronchial epithelial cells and eosinophils alone, IL6 release exhibited a synergistic effects in coculture of the two kinds of cells in a well separated into up and down chambers by the screen, that could sustain the culture supernatant across ［(107±10) ng/L vs (388±40) ng/L, 0.001］. But IL6 release in coculture of the two kinds of cells separated using screen was less than that in their contact coculture ［(388±40) ng/L vs (1270±131) ng/L, 0.001)］. Coculture of bronchial epithelial cells and eosinophils in Matrigel system led to asignificant elevation of IL6 release compared with that in general contact coculture of the two kinds of cells ［(2076±165) ng/L vs (1270±131) ng/L, 0.01)］. CONCLUSION: Human bronchial epithelial cells and eosinophils can be activated to release IL6 by contacting each other and mediators that they released in coculture. 【Keywords】 bronchi epithelial cells eosinophils interleukin6 【摘要】 目的： 以支气管上皮细胞和嗜酸粒细胞共培养为实验模型，探讨不同培养方式对IL6释放的影响. 方法： 将支气管上皮细胞和嗜酸粒细胞分别单独培养、接触共同培养、分隔共同培养（用分隔膜将两种细胞分隔在上、下两个池中不接触培养，两池中的培养液可以自由通过分隔膜孔）和在Matrigel中共同培养. 两种细胞在不同培养条件下释放的IL6应用酶联免疫吸附试验方法定量. 结果： 支气管上皮细皮与嗜酸粒细胞在同一培养孔中分隔共同培养时其IL6释放量远高于两种细胞分别在两个培养孔中独自培养后上清液中IL6之和［(388±40) ng/L vs (107±10) ng/L, 0.001］.而两种细胞在同一培养孔中（没有隔膜分隔）接触共同培养，其IL6释放量又远高于其分隔培养［(1270±131) ng/L vs (388±40) ng/L, 0.001］；将上述两种细胞在Matrigel中共培养,其IL6释放又显著高于其在非Matrigel中的培养 ［(2076±165) ng/L vs (1270±131)