持续动态压应力下骨折愈合时环氧化酶及相关信号分子的表达_医学论文.docVIP

持续动态压应力下骨折愈合时环氧化酶及相关信号分子的表达_医学论文 持续动态压应力下骨折愈合时环氧化酶及相关信号分子的表达_医学论文 作者：任可 张春才 汪光晔 赵建宁 陆维举 李波 【摘要】 　　目的：探讨持续动态压应力环境对实验性骨折愈合时环氧化酶mRNA表达水平以及前列腺素E2 (PGE2)和环磷酸腺苷(cAMP)含量的影响. 方法：80只新西兰兔行单侧肱骨干横断截骨，实验组以天鹅记忆接骨器内固定，在骨折端造成持续动态压应力，对照组以4孔加压钢板固定. 分别于术后第3， 7， 14， 21， 28， 56， 84日时，在骨折间隙内取材，行组织学、放射免疫和Realtime RTPCR等观察及检测. 结果：环氧化酶的表达量以及PGE2和cAMP的含量均较骨折前显著上升，且实验组在术后3~4 wk时以上指标明显高于对照组，组织学结果则显示实验组骨折间隙内软骨内成骨和编织骨向板层骨的改建均领先于对照组. 结论：天鹅记忆接骨器产生的持续动态压应力通过COX，PGE2和cAMP这一信号通路使得成骨细胞分化成熟，促进了骨折愈合. 【关键词】 骨折内固定术； 骨折愈合； 应力； 环氧化酶； 前列腺素E2； 形状记忆合金 　　【Abstract】 AIM: To explore the effect of continuous dynamic pressure stress on cyclooxygenase(COX) mRNA expression and prostaglandin E2 (PGE2) and cAMP contents during experimental fracture healing. METHODS: Unilateral osteotomy of left humeral diaphysis was performed in 80 adult New Zealand rabbits. The humeral fracture was fixed with swanlike memory connector (SMC) in study group and with 4hole dynamic compression plate (DCP) in control group. Samples from the fracture gaps were obtained at 3, 7, 14, 21, 28, 56 and 84 days after operation. The temporal expression patterns of COX mRNA during the repair process were examined through realtime RTPCR. The contents of PGE2 and cAMP were detected through radioimmunity method and the process and quality of fracture healing were observed histologically. RESULTS: The relative levels of COX2 mRNA as well as the contents of PGE2 and cAMP were elevated after fracture, and were significantly higher in study group than those in control group at 3 and 4 weeks postoperatively. Histological observation revealed that the mineralization of cartilage matrix, the endochondral bone formation and the callus remodelling took place earlier in study group. CONCLUSION: The persistent dynamic longitudinal pressure stress produced by SMC may contribute to endochondral bone formation of callus and accelerate fracture healing, which is considered to be closely related with the signaling pathway that has several critical components